Hello, I am working with imaging in larval zebrafish. I am interested in looking into the expression patterns of a particular protein in real time along their intestine in response to lipid consumption. The problem I am having designing this experiment is that the larva are too small to be fed without having them immersed in a mixture of EM and lipid. I want to watch the feeding and expression as close to real time as possible, preferably from the moment the lipids enter the intestine. I need to work while the fish are still transparent for the imaging to work like I want it to. Does anyone know if it is possible to feed the fish while they are immobilized on a imaging platform. Thanks in advance
Andrew
I am not sure but you can try with micro injector along with magnifier glass
There are some papers,where you can microgavage the choice of food directly into the intestine.I also do microgavage and it is similar like microinjections,But little difficult than that.You should check the protocol.
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