Fraction collector Frac-920 not working with AKTA?

Hi, I routinely use AKTA connected to Fraction Collector-920 for collecting proteins while doing protein purification. However, recently I have started seeing some errors on our Fraction...

27 February 2020 9,025 4 View

No colonies from NEB high-efficiency chemically competent cells?

Hi all, I am doing a few site-directed mutagenesis reactions and after digestion of the parent plasmid using Dpn1, I add the complete 50 uL reaction volume to 50 uL of NEB chemically competent...

01 November 2019 3,085 3 View

Low plasmid miniprep yields?

Hi, I have been working on plasmid minipreps using Qiagen miniprep kit and I have run into weird problems of low plasmid yields. When we purchased a new kit, then my plasmid concentrations were...

12 March 2019 9,028 3 View

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats?

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats when compared to Control rats?

14 September 2018 6,964 2 View

How to prepare simulated distillery wastewater in laboratory?

I want to check the bio-remediation of distillery wastewater.

10 June 2018 1,340 3 View

Does anyone have expertise using Getis-Ord hotspot analysis?

14 March 2017 541 2 View

Calculating nanomoles with knowing only conc and OD?

Hi All, I am wondering if we can calculate the nanomoles of RNAi, knowing only details of its ug/ul concentration and OD values; though no information of basepair is available? please suggest! Best,

02 February 2017 432 3 View

Is there any empirical relation to deduce mineral N in the soil layer on the basis of Available nitrogen?

Mineral N (nitrate and ammonical form) is highly dynamic, and is generally not measured by most of the researchers, who most of the time take total N or available N in consideration for evaluating...

28 January 2016 6,998 5 View

Several soil quality indices reported in literature, but effective operational soil health service missing. Please suggest the ways.

1. Lot of information on soil test values (spatio-temporal)) available 2. Soil quality indices e.g. SQI by Ratan Lal et al, Cornell soil health index, STCR network ar Indian Institute of Soil...

21 January 2016 8,570 26 View

What is the process of converting HB (harwell boeing) matrix to sparse matrix?

To get the stiffness matrix of a FE model from ANSYS to MATLAB I converted it into HB format. Now I want to convert it into sparse format using MATLAB. Is there any program in MATLAB to do so. If...

13 January 2015 369 3 View

What are the ranges of four diffterent poles of butterworth filter for ECG signal?

Hi, Can anyone please tell me what are the ranges of four different pole orders such as first order, second order, third order and fourth order respectively of butterworth filter for ECG...

23 February 2021 2,554 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to do a surface treatment of uncoated TiO2 with polymer?

Basically, I would like to know how to treat or coat on the uncoated bare TiO2 (Ti-OH rich) surface with polymer which has anchoring groups in the polymer structure like trimethoxygroups. What is...

21 February 2021 4,657 6 View

How can I measure the surface interaction or surface interfacial chemistry between polishing abrasives and upper surface of substrate ?

I am working on polishing abrasives. I need to do few characterizations ? which can provide the on time surface chemistry of substrate with polishing abrasive during optical polishing. I would...

18 February 2021 5,319 3 View

Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

How to detect uv vis spectrum of nanoparticle under 300nm?

I try to make the indium nanoparticle and check the uv vis spectrum. I heard that In nanoparticle has surface plasmon resonance peak at 200~300nm. But most of solvent has absorption peak under...

14 February 2021 6,950 15 View

Which is the best silane for making a borosilicate glass surface hydrophobic permanently? and what is the protocol?

Regarding my needs I am looking for a glass coating agent that makes the glass hydrophobic permanently

09 February 2021 3,924 5 View

What is the difference between Graphene Oxide and Expanded Graphite?

What are the main differences between Graphene Oxide and Expanded graphite, in terms of surface functionalities, conductivity, d-spacing and other features? Is the term expanded graphite...

06 February 2021 9,810 4 View

How to eliminate noise when using a sensor with instrumentation amplifier and giving it's output to Arduino?

When I am trying to implement a pressure sensor through instrumentation amplifier and then giving it's output to Arduino, I am getting garbage value due to noise, so how to eliminate it.

05 February 2021 8,598 11 View