How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View

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02 December 2020 7,293 2 View

What is the physical meaning of the cross term of the index performance of LQR ?

Dear All : I have a curious question. Among the index performance of the LQR, X' Q X and u' R u have the physical meaning. Now, my question what is the physical meaning of the cross term X' S...

02 August 2020 1,780 1 View

Can anyone help me about probability sampling?

Now I am doing my DBA research, the whole population are the public university teachers in one of Chinese 's province, there are 22 universities. and I want 400 samplings, the total population is...

23 February 2020 248 3 View

On-market phage resistant E.coli strains for pr expression?

ER2566 by NEB is what we are utilizing for pr expression, with merits of fhuA2 mutation. Apparently its resistance spectrum is not wide enough to combat all kinds of bacteriophages we encounter,...

28 December 2019 3,211 3 View

3'deoxy ribonucleotide available?

Hi, I wonder if people know 3'deoxy ribonucleotide (i.e. with 2'OH) is commercially available? I didn't find any in the modified bases product section in IDT, thermo, or sigma. I'd like to use...

14 October 2019 8,376 3 View

Has a researcher done or is doing research on the photoinitiator tetraethylmethane ketone?

Dear friends, why use tetraethylmethane ketone as photoinitiator (5wt% of photomonomer added), triethanolamine as co-initiator, UV-induced (light wavelength 365nm) acrylamide double bond...

17 April 2019 9,857 1 View

How to quantify amyloid load of IHC staining images with Image J?

Hi, everyone, I would like to quantify the Abeta load of my IHC staining images. How should I do it? someone use plaque area %, someone use plaque numbers, which one is better? And can I do it...

15 February 2019 2,109 1 View

How many dose can we use in radiotherapy?

Hi We designed a radiotherapy study, in this experiment we used hypofraction therapy that 4 Gy per day treated local tumor and gave 4 days, the total dose is 16 Gy. However, we observed 3 of 6...

11 May 2018 6,966 3 View

How to compare effect size between two items?

Dear all experts, I'm currently doing a fMRI study and compare two groups in two conditions (switch and non-switch), and wondering if they have differences in one ROI (region of...

18 April 2018 2,789 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

What is significance of Powder XRD peak in diamond like material?

Hi researchers! I would like to get your suggestions on powder XRD data. Recently I found the peak (100 % Relative intensity) at 2theta= 36 degree in diamond film in addition to peak at 2theta= 44...

21 January 2021 2,361 2 View

SDS-PAGE aggregation problem?

I ran SDS-PAGE with 14% lower gel, 4 % upper gel, having brushing, pre-running empty gel for 30 min. The protein samples had been centrifuged with 14,000g for 10 min, and then the supernatants...

20 January 2021 172 3 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View

Do you have information about synthetic diamonds and their environmental impact?

I am doing a deep research on the comparison of the process of extraction of natural diamonds and synthetic diamonds, and their environmental impact I would appreciate you could share your...

19 January 2021 1,359 3 View

Best loss function for special classification problem in neural networks ?

Hello, I've build a Conv1D model to classify items into 6 categories (from 0 to 5). (simplified example) I use therefore a CrossEntropy loss function that penalizes the errors with high...

19 January 2021 3,199 1 View

Can anyone suggest a commercial MAT kit to detect Toxoplasma gondii antibodies in animals?

18 January 2021 5,438 2 View

What percentage torque ripple exists in electrical motors used in electric vehicles ?

I am trying to understand the significance/percentage of torque ripples that exist in electrical motors (under operating conditions) that are used in commercial electric vehicles. If someone could...

15 January 2021 5,691 3 View