Do DMSO effect the microoragnisms, if so what would be the minimum concentration to exhibit activity?
Dimethylsulfoxide (DMSO) may bind within the cytoplasmic membrane and increase its permeability.
Hi, the solvent DMSO not inhibit growth of microorganisms ..I think (2.5%) not effect. But high concentration may increase permeability of cytoplasm membrane of microorganism and give false results.
DMSO can inhibit microbial growth. The concentration will vary depending on the organism. Dr Chanders pdf is a good place to start
Hi Akshatha!
When I was working in research about antimicrobial activity of vegetal compounds, we assessed activity of esential oils by well diffusion in agar plates inoculated with bacteria using a cotton swab. Oils were diluted in DMSO and water and 100% DMSO was used as control.
I never saw growth inhibition against E. coli, S.aureus, B. subtilis and P. aeruginosa but we never tried with diluted DMSO.
Best regards.
Hi Akshatha!
First of all you should test this yourself by conducting a pilot study on one gram positive and on gram negative bacterial strain using different concentrations of DMSO including with the concentration you are looking for your final experiment. you can do that in just a single experiment using two MH agar plates after lowing of overnight bacterial culture (Gram positive and Gram negative) on each plate respectively) making wells on plates, by labeling the wells accordingly and purring of DMSO to each well with respect to its concentration. you can check it out next day after overnight incubation. More over you can use propylene glycol as solvent for non polar compounds as it has found with no inhibitory effect in many peer reviewed publications.
Generally less than 1% should be fine and expected not to interfere with the microbial growth.
yes, it has an antimicrobial effect against some strains, so you should use control to set the less dosage.
I don't think DMSO show any activity, but it;s better to run a control of DMSO for any inconvenience later.
Thanks
I used 100% DMSO against S. aureus, E. coli, A. flavus, but there was no activity against any of the microbes.
I used 100%DMSO against S. aureus did not show any inhibition but at 50% dilution showed some activity
What is the final percentage of DMSO will determine whether it will have any effect ok bacterial growth. For example, If you add 50 uL bacterial culture to 50 uL 100% DMSO, your bacterial should never grow.
I agree with you my dear Rabi Sanusi Zayyan.
Please kindly may you send some references to support that "I used 100% DMSO against S. aureus, E. coli, A. flavus, but there was no activity against any of the microbes." Thanks in advance.
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