Alejandro Piña Iturbe

2 Questions 12 Answers 0 Followers

Questions related from Alejandro Piña Iturbe

Why my transformed BL21(DE3) stops growing at OD600=0.55-0.6?

Hi everyone! Previously, I transformed a BL21(DE3) strain with a pET-15b vector carrying my gene of interest. The strain grew and I could express my protein. Now I need to do that again, but...

22 April 2019 6,608 4 View

In which growth phase are bacteria in their natural environment?

Hello everyone! Usually in the lab, growth curves are constructed for different bacterial species, and the effect of different treatments or stimuli, and the changes in proteins and mRNA are...

01 January 1970 4,250 1 View