Mubasher Rehman

13 Questions 41 Answers 0 Followers

Questions related from Mubasher Rehman

In ELISA should we use p-nitrophenyl phosphate (pNPP) substrate mixed in distilled water or we should mix it in Diethanolamine buffer?

We are performing an ELISA experiment to determine the binding of Complement antibodies against P. aeruginosa. We have pNPP substrate (Thermo Scientific cat # 34045) instead of pNPP tablets. We...

23 December 2020 2,194 3 View

Ibuprofen is associated with COVID-19 death, is it true?

If true it must relate to how ibuprofen's mechanism of action occurs to block inflammation & this the immune system cascade. It may block the primary paths the body needs for immune system to...

29 March 2020 2,700 6 View

What is the difference between skim milk and Bovine serum albumin for primary antibody in western blotting/ELISA??

I want to investigate role of complement antibodies (C1q, MBL, CL-11 etc) against Pseudomonas aeruginosa. For this research work i have to use BSA for blocking purpose but can i use skim milk...

13 March 2020 1,218 10 View

Can we add glucose for making M9 minimal media containing C6-HSLs as a sole source of carbon?

I am workimg on isolation of quorum quenching bacteria from soil. I want to use M9 minimal media containing homoserine lactones as a sole source of carbon. Therefore, can we add glucose too into...

15 October 2019 4,828 2 View

Which method is best for serotyping of campylobacter spp isloated from chicken wastes?

According to a study (by Dorothy McKay et al. Link of paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC88048/ ) two methods are widely used for serotyping of campylobacter spp; 1. Penner...

18 September 2019 8,770 6 View

Creating new group for sharing life sciences research ideas

Hi, I am thinking to create a group (on Whatsapp/Facebook or on any other application) of students, teachers, and mentors in which we all will share our ideas that how we can discover new things...

01 January 1970 605 12 View

Immunotherapy vs antibiotics vs Phage therapy? Which one you prefer & why (

The use of complement system/immunotherapy or antibiotocs or phage therapy to treat microbial diseases. We all know that resistance are increasing day by day specially in case of antibiotic then...

01 January 1970 3,408 5 View

Can any one please suggest me journal accepting review articles on COVID-19?

I am seeking for clarivate index journal (peer reviewed JCR listed journal) which is accepting review article on COVID-19 in its special issue. Thank you

01 January 1970 1,358 10 View

What are the benefits of joining any society or organization, specially microbiology society?

Would it be helpful in future job or academically? If yes then which one is best & why? if no then what are the reasons? Thanks

01 January 1970 3,451 5 View

How to open a journal?

Hi, Can anybody let me know the basic requirements for opening a journal? What are the required documents & how much does it cost? And how we can include it in JCR list? Thanks

01 January 1970 6,604 2 View

Delay in degree completion because of COVID-19 pandemic & it's effect?

As because of COVID-19 pandemic, worldwide degree will be delayed (specially MPhil degree probably will take at least 5 semesters). Will it have any effect on future job or PhD?

01 January 1970 797 7 View

Immunology & artificial intelligence?

Hi, I want to start a very interesting discussion, please take part if you think same. Can we use artificial intelligence in Immunology? Like creating diagnostic kits, experiments & many...

01 January 1970 8,271 5 View

As scientists are more focusing on phage therapy to replace antibiotics but what if phage resistance occurs in future?

A few decades ago, when scientists discovered the way to treat an infection through antibiotics, it was remarkable and were appreciated worldwide but now a day’s worldwide problem is antibiotic...

01 January 1970 4,984 6 View