In DBT-CTEP travel grant, do the actual travel dates must match the dates mentioned in Fare certificate submitted along with the application form?

Hi, I have been awarded the DBT-CTEP travel grant to attend the conference "64th Annual Meeting of the Biophysical Society" in San Diego which is going to be held between 15th to 19th February,...

22 January 2020 6,490 1 View

Does having water between wells on 96 well plate affect cell growth in wells?

After setting up 100 uL of 10000 cells/mL of the HCT-116 colorectal cancer cell line, we notice that when we place 40 uL of water in the pockets between wells, that our cells grow to about half...

15 August 2018 902 2 View

Why does my observation differ from the literature in RBC stiffness measurement?

I am trying to measure stiffness of red blood cells in RPMI using AFM force spectroscopy method. In literature, the typical indentation of RBC at a force of 500pN is 500nm. But in my case I see...

10 July 2018 7,831 5 View

Why does RBC crenate after attaching to glass surface?

I am performing AFM force spectroscopy on red blood cells by immobilizing them on glass surface. I clean the glass coverslips by sonication in soap solution, Milli Q, ethanol, acetone, ethanol for...

25 March 2018 683 3 View

Can we perform PCR with a primer which is thiolated?

I would like to prepared a DNA sample that has -SH or thiol group at one of its end so that I can covalently immobilize it on a surface. The -SH modified primers are commercially available. I am...

19 December 2017 5,610 6 View

Can we image DNA using scanning electron microscope?

I would like to attach DNA to AFM tip and image it using SEM to check if coating was done properly. I couldn't find any papers that show SEM images of DNA. I would like to know the protocol to do...

12 November 2017 4,241 2 View

How do I check if core histones are present in octameric form?

I have purchased human histone octamers from Sigma (SRP0408) for nucleosome reconstitution experiments. I would like to check the protein complex in octameric form. Running it on SDS-PAGE will...

30 October 2017 3,315 5 View

Regarding the flowrate using Harvard apparatus syringe pump?

I am using syringe pump with the flowrate set to 5ul/min. When I flow water which is in plastic syringe through a 1mm 0.5mm tube and collect the liquid for 10min. Then I see that ~50ul liquid is...

25 July 2017 2,624 2 View

Does the DNA purity matter for nucleosome reconstitution experiment?

I would like to prepare mono nucleosomes for my single molecule experiments. We have PGEM 3Z vector (size is 3kb) from which we are amplifying the 344bp oligo that contains the Widom 601 sequence...

14 July 2017 9,037 1 View

Which is the best anhydrous organic solvent?

I am trying to perform APTES coating on glass coverslips and AFM tips. I need the most anhydrous organic solvent to dissolve APTES so that there wont be any polymerization of APTES. I have seen...

03 July 2017 4,587 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...

01 March 2021 8,415 1 View

How to increase mesh quality of a cfd model in ansys fluent?

How can one improve mesh skewness, orthogonality, aspect ratio?

28 February 2021 7,730 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

How to extract the y+ value from fluent ?

I am doing a 3D steady simulation of a wind rotor using Ansys Fluent. In order to have a y+ of 1, I created 10 inflation layers using total layer thickness method, and I started a first...

23 February 2021 2,819 3 View

Help with assigning pressure boundary conditions in FLUENT on a large structure?

Hello, I created a very tall tower (>1000m) and applied pressure boundary conditions at the inlet (bottom) and outlet (top) that correspond to the pressure are their relative altitudes....

22 February 2021 9,972 4 View

How do I add a udf to Ansys fluent and does it need to be compiled before hand ?

I have a Define_CG_Motion udf written in c where do I add it to my Ansys fluent project and does it need to be compiled before that?

21 February 2021 9,347 3 View

How do I plot Pressure against crank angle degree in Fluent?

18 February 2021 8,390 3 View