In DBT-CTEP travel grant, do the actual travel dates must match the dates mentioned in Fare certificate submitted along with the application form?

Hi, I have been awarded the DBT-CTEP travel grant to attend the conference "64th Annual Meeting of the Biophysical Society" in San Diego which is going to be held between 15th to 19th February,...

22 January 2020 6,490 1 View

Does having water between wells on 96 well plate affect cell growth in wells?

After setting up 100 uL of 10000 cells/mL of the HCT-116 colorectal cancer cell line, we notice that when we place 40 uL of water in the pockets between wells, that our cells grow to about half...

15 August 2018 902 2 View

Why does my observation differ from the literature in RBC stiffness measurement?

I am trying to measure stiffness of red blood cells in RPMI using AFM force spectroscopy method. In literature, the typical indentation of RBC at a force of 500pN is 500nm. But in my case I see...

10 July 2018 7,831 5 View

Why does RBC crenate after attaching to glass surface?

I am performing AFM force spectroscopy on red blood cells by immobilizing them on glass surface. I clean the glass coverslips by sonication in soap solution, Milli Q, ethanol, acetone, ethanol for...

25 March 2018 683 3 View

Can we perform PCR with a primer which is thiolated?

I would like to prepared a DNA sample that has -SH or thiol group at one of its end so that I can covalently immobilize it on a surface. The -SH modified primers are commercially available. I am...

19 December 2017 5,610 6 View

Can we image DNA using scanning electron microscope?

I would like to attach DNA to AFM tip and image it using SEM to check if coating was done properly. I couldn't find any papers that show SEM images of DNA. I would like to know the protocol to do...

12 November 2017 4,241 2 View

How do I check if core histones are present in octameric form?

I have purchased human histone octamers from Sigma (SRP0408) for nucleosome reconstitution experiments. I would like to check the protein complex in octameric form. Running it on SDS-PAGE will...

30 October 2017 3,315 5 View

Regarding the flowrate using Harvard apparatus syringe pump?

I am using syringe pump with the flowrate set to 5ul/min. When I flow water which is in plastic syringe through a 1mm 0.5mm tube and collect the liquid for 10min. Then I see that ~50ul liquid is...

25 July 2017 2,624 2 View

Does the DNA purity matter for nucleosome reconstitution experiment?

I would like to prepare mono nucleosomes for my single molecule experiments. We have PGEM 3Z vector (size is 3kb) from which we are amplifying the 344bp oligo that contains the Widom 601 sequence...

14 July 2017 9,037 1 View

Which is the best anhydrous organic solvent?

I am trying to perform APTES coating on glass coverslips and AFM tips. I need the most anhydrous organic solvent to dissolve APTES so that there wont be any polymerization of APTES. I have seen...

03 July 2017 4,587 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View

Does anyone know of a predictable relationship beween surface gravity and atmospheric composition?

The attached file demonstrates that there is a predictable relationship between the structural density of a terrestrial body and its none-rotating surface gravity. Using Newton's gravity law of...

26 February 2021 2,317 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View