10 Questions 7 Answers 0 Followers
Questions related from Bora Onat
Hello everyone, I'm having trouble detaching peripheral blood mononuclear cells (PBMCs) we got from ATCC out of a TC-treated plate and even a Greiner suspension plate. I need to do an enrichment...
03 March 2025 9,936 2 View
Hello everyone, We have a Dionex LC that we control through Chromeleon 6.8 and a Waters QTof that is managed through MassLynx. Both are old instruments, we only have these two software and are...
21 June 2021 363 1 View
Hello everyone, I've been trying to resurrect an old Dionex/LC Packings HPLC which used to use Chromeleon 6.7. We have the license key for this software, but somehow the CD got lost while we were...
18 June 2021 4,349 5 View
Hello everyone, We have a Waters Micromass QTof Micro system, which is probably about 20 years old, and we're trying to resurrect it. Currently, it looks like the pump, voltage controls, ESI...
14 June 2021 3,242 4 View
Hello there, I know that shotgun proteomics relies on high-resolution instruments (i.e. Orbitrap and New QTof systems) with nano-LC flow and nano-ESI mostly, however I only have a reach to a...
09 June 2021 2,446 3 View
I have a list of about 600 metabolites that I'd like to find the possible metabolic pathways for. The only way to search the pathways they are involved in is to submit their names to the KEGG...
12 June 2020 8,444 0 View
Hello all, We are currently working on angiotensin analysis, however we observed that, once the freeze-dried peptide is dissolved in an aqueous buffer, it gets unstable in only 2-3 hours. Mass...
14 November 2019 1,006 1 View
Dear all, I use Saos-2 osteoblast-like cancer cells on my research to assess the osteoconductive and osteoinductive properties of some biomaterials. Saos-2 cells divide great and their viability...
01 October 2016 7,337 7 View
To induce the diiferentiation of SaOS-2 cells, researchers suggest using L-ascorbic acid, dexametasone and beta-glycerophosphate. However, unfortunatelly, beta-glycerophosphate has not arrived...
05 March 2016 8,292 0 View
Hello everyone, I was wondering, does anyone have experience in transferring a cryovial of a cell line from Canada to another country? I know there are companies who do this for profit, but is it...
01 January 1970 2,028 3 View
