I see a new term on RG statistics page "Total Research Interest":What does this interpret?

"Total Research Interest" is some new index. Can someone explain what does this imply? Maybe its a simple entity but iam not aware of this. Regards

01 February 2019 2,837 26 View

No title

31 December 2018 1,881 30 View

Should basic medical college training other allied medical specialties incorporate managerial sciences and information technologies?

The world is changing fast and lot many biotechnologies are now being routinely utilized by medical professionals in all domains. However, they do not have a formal training in this regard....

10 November 2018 2,917 24 View

How do you compare glucometer readings with lab gkucose results?

While both glcometer & lab glucose measuring technologies use different methodologies and sample. Still from physician to medic compare their results. What do you trust more & for what...

10 November 2018 5,017 7 View

I was looking at the publication fee before depositing an article for review. Can anyone explain what these terms mean?

Dear ALL Creative Commons Attribution (CC BY) Creative Commons Attribution-Non Commercial-No Derivs (CC BY-NC-ND) I am not sure what these publication options means.Obviously i intend to pay...

10 November 2018 1,860 16 View

Do you think genomic medicine will replace conventional medicine ?

Genomic data is emerging fast and every something new molecular research data is there. So what is you take on my question?

10 November 2018 7,222 23 View

Should we as healthcare worker waer our white coats/hospital dress outside the hospital?

I believe we are spreading more infection and antibiotic resistance if we are taking/wearing our white coats (the trade mark of ant medic) outside hospital. Should we do that? Are we not spreading...

09 October 2018 3,555 15 View

What are the differences between miRNA,siRNA, piwiRNA and lncRNA? ?

I will appreciate if u give specific differences between siRNA and miRNA and other types of RNAs. If anybody could elaborate. Regards Dr Sikandar

08 September 2018 5,545 2 View

If you are writing a mini review can you add a scematic from somebody's article after referencing?

This question is about writing a mini review where i want to add a schematic from some article after appropriate referencing.Can i do that?

08 September 2018 5,590 4 View

What is the preferenable bioniformatics base for RNA base alignments?

Google search and different RNASeq experiments highlights many types of RNA bases for read alignments. Can someone suggest a user friendly base fro small micro RNA alignments to known sequences?

07 August 2018 3,011 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View