Anbody being aware of/knowledge of/ done single molecule sequencing?

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I see a new term on RG statistics page "Total Research Interest":What does this interpret?

"Total Research Interest" is some new index. Can someone explain what does this imply? Maybe its a simple entity but iam not aware of this. Regards

01 February 2019 3,028 26 View

What is the difference Between “Contributor”, “Co-Author” and "Collaborator" in authorship list? Does all 3 categories merit as authors for research?

"Publish or perish".....there is like an ongoing competition to publish more, I guess more will acknowledge that. What i need to know is the difference Between “Contributor”, “Co-Author” and...

31 December 2018 2,438 30 View

How do you compare glucometer readings with lab gkucose results?

While both glcometer & lab glucose measuring technologies use different methodologies and sample. Still from physician to medic compare their results. What do you trust more & for what...

10 November 2018 5,189 7 View

Should basic medical college training other allied medical specialties incorporate managerial sciences and information technologies?

The world is changing fast and lot many biotechnologies are now being routinely utilized by medical professionals in all domains. However, they do not have a formal training in this regard....

10 November 2018 3,087 24 View

I was looking at the publication fee before depositing an article for review. Can anyone explain what these terms mean?

Dear ALL Creative Commons Attribution (CC BY) Creative Commons Attribution-Non Commercial-No Derivs (CC BY-NC-ND) I am not sure what these publication options means.Obviously i intend to pay...

10 November 2018 2,029 16 View

Do you think genomic medicine will replace conventional medicine ?

Genomic data is emerging fast and every something new molecular research data is there. So what is you take on my question?

10 November 2018 7,373 23 View

Should we as healthcare worker waer our white coats/hospital dress outside the hospital?

I believe we are spreading more infection and antibiotic resistance if we are taking/wearing our white coats (the trade mark of ant medic) outside hospital. Should we do that? Are we not spreading...

09 October 2018 3,729 15 View

What are the differences between miRNA,siRNA, piwiRNA and lncRNA? ?

I will appreciate if u give specific differences between siRNA and miRNA and other types of RNAs. If anybody could elaborate. Regards Dr Sikandar

08 September 2018 5,796 2 View

If you are writing a mini review can you add a scematic from somebody's article after referencing?

This question is about writing a mini review where i want to add a schematic from some article after appropriate referencing.Can i do that?

08 September 2018 5,749 4 View

What is the preferenable bioniformatics base for RNA base alignments?

Google search and different RNASeq experiments highlights many types of RNA bases for read alignments. Can someone suggest a user friendly base fro small micro RNA alignments to known sequences?

07 August 2018 3,168 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

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