Hi, so I have trouble analysing the data from the light cycler 480 II Dual color hydrolysis probe / UPL probe for trying a COVID kit from genesis. I did a standard curve using a internal positive control (CPT), the standard curve looks beautiful (dilution up to 10000), and then i tested 8 samples, they all show some amplification Cp, but pretty low and quite similar to the highest dilution of positive control.

I exported the data as .txt file but then how do I "normalised" the unknown samples to be tested, relative to the CPT? How do I do that exactly?

Could someone give me some advices?

Many many thanks in advance.