Isolation and identification of proteins?

I am looking for the protein expression of Methanosarcina mazeii that can get expressed in the presence of acetate. I am not much aware of steps. I was planning to do cell-lysis and then SDS-PAGE...

01 February 2019 6,089 12 View

White things present in anaerobic culture bottle with coal powder as a substrate?

I am getting a white crystalline things in my anaerobic culture bottles. I have used same medium from a year and this never happened before. Can anyone suggest me, what this could be? Just for...

10 November 2018 5,340 1 View

What about "Journal for Negative Results"?

Hello everyone, wish you all a very Happy and Prosperous New Year 2015. This is regarding a journal that can have all negative results. We all are doing enormous numbers of experiments and getting...

31 December 2014 217 25 View

Can someone help me with Protein Purification using Ni-NTA?

I am working on a protein, that I tried to express in BL-21 cells. This protein is an integral membrane protein, so I tried harsh buffer conditions to take out this protein in soluble form. The...

11 December 2014 8,963 24 View

What are the ways to use thymoquinone in animals for breast cancer studies?

I have achieved 90 % killing in-vitro on MCF-7 and now I am proceeding in-vivo. I will be using BALB c mice for studies. I will inject 4T1 (mouse myeloma) that mimics breast cancer. What are the...

07 August 2013 5,374 1 View

Can anyone suggest the concentrations of Thymoquinone to be used anticancer activity research and recommend some articles?

I have used 25uM to 200uM concentrations of Thymoquinone (TQ) on MCF-7 (Breast Carcinoma), with 24 hours of incubation, but I didn't get proper cytotoxicity, I am going to increase incubation time...

06 July 2013 702 8 View

Can someone advise on using animal models for prostate cancer?

Can we use normal mice for study of prostate cancer? And what other animal models can be used for prostate cancer as well as breast cancer.?

06 July 2013 4,689 2 View

MTT Assay

Is there any substitute for MTT? I have to do an MTT assay but I am unable to find it!

06 July 2013 2,389 8 View

I have to work on 4 T-1 but its not reviving, can you help me?

I joined a new lab and I have to start work on 4T-1, but its not reviving. I don't understand, we are using RPMI 15%. My new lab mates freezed the cells and after adding freezing media they kept...

05 June 2013 4,213 8 View

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04 May 2013 9,321 22 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View