Anaerobic serum bottles pressure range?

Does anyone know the range of pressure that 120ml anaerobic serum bottles can tolerate (10-100 psi)?

01 February 2019 2,764 3 View

Isolation and identification of proteins?

I am looking for the protein expression of Methanosarcina mazeii that can get expressed in the presence of acetate. I am not much aware of steps. I was planning to do cell-lysis and then SDS-PAGE...

01 February 2019 6,089 12 View

White things present in anaerobic culture bottle with coal powder as a substrate?

I am getting a white crystalline things in my anaerobic culture bottles. I have used same medium from a year and this never happened before. Can anyone suggest me, what this could be? Just for...

10 November 2018 5,340 1 View

What about "Journal for Negative Results"?

Hello everyone, wish you all a very Happy and Prosperous New Year 2015. This is regarding a journal that can have all negative results. We all are doing enormous numbers of experiments and getting...

31 December 2014 217 25 View

Can someone help me with Protein Purification using Ni-NTA?

I am working on a protein, that I tried to express in BL-21 cells. This protein is an integral membrane protein, so I tried harsh buffer conditions to take out this protein in soluble form. The...

11 December 2014 8,963 24 View

What are the ways to use thymoquinone in animals for breast cancer studies?

I have achieved 90 % killing in-vitro on MCF-7 and now I am proceeding in-vivo. I will be using BALB c mice for studies. I will inject 4T1 (mouse myeloma) that mimics breast cancer. What are the...

07 August 2013 5,374 1 View

Can anyone suggest the concentrations of Thymoquinone to be used anticancer activity research and recommend some articles?

I have used 25uM to 200uM concentrations of Thymoquinone (TQ) on MCF-7 (Breast Carcinoma), with 24 hours of incubation, but I didn't get proper cytotoxicity, I am going to increase incubation time...

06 July 2013 702 8 View

MTT Assay

Is there any substitute for MTT? I have to do an MTT assay but I am unable to find it!

06 July 2013 2,389 8 View

I have to work on 4 T-1 but its not reviving, can you help me?

I joined a new lab and I have to start work on 4T-1, but its not reviving. I don't understand, we are using RPMI 15%. My new lab mates freezed the cells and after adding freezing media they kept...

05 June 2013 4,213 8 View

No title

04 May 2013 9,321 22 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View