Priyanka Srivastava

14 Questions 51 Answers 0 Followers

Questions related from Priyanka Srivastava

Isolation and identification of proteins?

I am looking for the protein expression of Methanosarcina mazeii that can get expressed in the presence of acetate. I am not much aware of steps. I was planning to do cell-lysis and then SDS-PAGE...

02 February 2019 6,183 12 View

Anaerobic serum bottles pressure range?

Does anyone know the range of pressure that 120ml anaerobic serum bottles can tolerate (10-100 psi)?

02 February 2019 2,887 3 View

White things present in anaerobic culture bottle with coal powder as a substrate?

I am getting a white crystalline things in my anaerobic culture bottles. I have used same medium from a year and this never happened before. Can anyone suggest me, what this could be? Just for...

11 November 2018 5,419 1 View

What about "Journal for Negative Results"?

Hello everyone, wish you all a very Happy and Prosperous New Year 2015. This is regarding a journal that can have all negative results. We all are doing enormous numbers of experiments and getting...

01 January 2015 293 25 View

Can someone help me with Protein Purification using Ni-NTA?

I am working on a protein, that I tried to express in BL-21 cells. This protein is an integral membrane protein, so I tried harsh buffer conditions to take out this protein in soluble form. The...

12 December 2014 9,047 24 View

What are the ways to use thymoquinone in animals for breast cancer studies?

I have achieved 90 % killing in-vitro on MCF-7 and now I am proceeding in-vivo. I will be using BALB c mice for studies. I will inject 4T1 (mouse myeloma) that mimics breast cancer. What are the...

08 August 2013 5,449 1 View

MTT Assay

Is there any substitute for MTT? I have to do an MTT assay but I am unable to find it!

07 July 2013 2,469 8 View

Can someone advise on using animal models for prostate cancer?

Can we use normal mice for study of prostate cancer? And what other animal models can be used for prostate cancer as well as breast cancer.?

07 July 2013 4,776 2 View

Can anyone suggest the concentrations of Thymoquinone to be used anticancer activity research and recommend some articles?

I have used 25uM to 200uM concentrations of Thymoquinone (TQ) on MCF-7 (Breast Carcinoma), with 24 hours of incubation, but I didn't get proper cytotoxicity, I am going to increase incubation time...

07 July 2013 787 8 View

I have to work on 4 T-1 but its not reviving, can you help me?

I joined a new lab and I have to start work on 4T-1, but its not reviving. I don't understand, we are using RPMI 15%. My new lab mates freezed the cells and after adding freezing media they kept...

06 June 2013 4,288 8 View

Shifting of cells from liquid nitrogen to -70°C

Liquid nitrogen was finished, so I shifted all cells into -70°C for few hours. Is this correct? Does anything happens with the cells?

05 May 2013 9,403 22 View

Can anyone give advice for plaque assay?

I am doing plaque assay on 6 wells plate, I usually allowed the virus to adhere on the cell surface for two hours, then addition of agarose overlay medium. I am using 200ul of inoculum. Where I am...

03 March 2013 8,611 12 View

What is the best method to purify protein of your interest from mixture of many proteins.

Proteins

12 December 2012 5,702 9 View

Is it possible to isolate virus from 9 days old inoculated flask?

What about the virus titre?

10 October 2012 4,843 17 View