I want to preserve urine samples. Can anyone suggest the volume of RNA later to be put in 6-7 ml urine sample ??
To preserve blood we use between 3 to 5 volumes of RNA later, for cultured cells resuspended in PBS - between 5 to 10 volumes. Obviously, when you want to use milliliters of sample, the volume of RNAlater becomes substantial. Maybe you could centrifuge your sample to pellet the cells and resuspend them in smaller volume of PBS?
I am a bit curious about your task here because somehow this question feels odd.
It is well known that the integrity of the cells in the urine depends upon the physicochemical conditions of the urine itself such as pH, density, temperature and the time elapsed between the collection of the urine specimen and the processing of the sample. Given the high susceptibility of ribonucleic acid (RNA) to undergo degradation, isolation of adequate quantities of high quality RNA from urinary cells is a challenging process and the difficulties are further enhanced when the samples have to be shipped from clinical centers to a central molecular core laboratory.
You can read this article:
http://www.gmo-qpcr-analysis.info/medeiros-rna-2003.pdf
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