I want to see the interaction of STAT3 & NFkB after IL-6 treatment by Co-IP?

06 July 2019 5,867 4 View

Is it any compensation issue or flow antibody titration or something else?

I stained four tubes: Tube 1: Unstained Tube 2: Stained with only Total STAT3 labelled with PerCP cy 5.5. Tube 3: Stained with only P-STAT3 (Y705) labelled with Alexa flour 647.(APC Chanel...

08 September 2018 6,496 4 View

RNA isolation kit from urine sample ?

Hiiii Can anyone advice regarding the best kit for RNA isolation from urine samples? I have used qiagen viral RNA kit but it gives vey high O.D ( ~ 3.1 ) I need purified RNA with good quality so...

02 March 2018 2,347 2 View

Amount of RNA later to preserve urine sample ?

I want to preserve urine samples. Can anyone suggest the volume of RNA later to be put in 6-7 ml urine sample ??

02 March 2018 4,629 2 View

Is there any other marker or antibodies apart from IL-17A (BD Biosciencs ) for the detection of IL-17 cells by Flow cytometry?

Hiii, For the detection of Th17 cells, I am using IL-17A antibodies from BD Biosciences in the patients with Hyper IgE syndrome.But my results (percentage of IL-17 cells) drastically vary from...

31 December 2017 4,494 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View