I want to see the RhoGTPase expression on VTA by the help of flowcytometry in mice model??
Are you looking for protocols for FC intracellular staining? Please check:
https://www.creative-diagnostics.com/flow-cytometry-protocol-intracellular-staining.htm
Dear All, I performed the Co-IP to see the binding and co-localization of STAT3: and NFkB p50 in Human PBMcs Co- immunoprecipitation were performed on PBMCs (4×106) from cells treated with and...
06 July 2019 5,965 4 View
I stained four tubes: Tube 1: Unstained Tube 2: Stained with only Total STAT3 labelled with PerCP cy 5.5. Tube 3: Stained with only P-STAT3 (Y705) labelled with Alexa flour 647.(APC Chanel...
08 September 2018 6,604 4 View
Hiiii Can anyone advice regarding the best kit for RNA isolation from urine samples? I have used qiagen viral RNA kit but it gives vey high O.D ( ~ 3.1 ) I need purified RNA with good quality so...
02 March 2018 2,446 2 View
I want to preserve urine samples. Can anyone suggest the volume of RNA later to be put in 6-7 ml urine sample ??
02 March 2018 4,831 2 View
Hiii, For the detection of Th17 cells, I am using IL-17A antibodies from BD Biosciences in the patients with Hyper IgE syndrome.But my results (percentage of IL-17 cells) drastically vary from...
31 December 2017 4,636 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View