5α-Androstan-17β-ol-3-one has a permit fee of $350 (DEA). I'm looking at effects of Estradiol on MSC and would like to investigate a male hormone aswell.
This link of artical may be useful
https://academic.oup.com/endo/article/147/5/2368/2501163
I use the NC3000 to mainly look at Adipose derived MSC. When comparing to the flow cytometer we get significantly different read outs.. It seems the NC3000 (staining with AO and DAPI) picks up a...
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Peripheral blood mononuclear cells will be used to track proliferation via CFSE in vitro. Stimulation via PMA to investigate mechanisms of immune dampening when co-cultured with MSC's.
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Can I use multiple donors for T cell assays? Will mixing cause unwanted stimulation?Peripheral blood mononuclear cells will be used to track proliferation via CFSE in vitro. Stimulation via PMA to...
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Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
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I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
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I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
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