I use the NC3000 to mainly look at Adipose derived MSC. When comparing to the flow cytometer we get significantly different read outs.. It seems the NC3000 (staining with AO and DAPI) picks up a smaller (less intense) population which it counts and feeds back as a higher cell count but less viable. When on the flow cytometer (FACScan and pretty old) (stain PI and SYTO11) these "cells" would be in the debris.
Does AO simply light up everything? I'm assuming these "cells" are DNA fragments that are just not being picked up on the flow we have.. Any suggestions besides get a better flow?
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