Flyn Mckinnirey

4 Questions 3 Answers 0 Followers

Questions related from Flyn Mckinnirey

Has anyone had any experience with the NC3000 Chemometec for assessing cell count and Viability?

I use the NC3000 to mainly look at Adipose derived MSC. When comparing to the flow cytometer we get significantly different read outs.. It seems the NC3000 (staining with AO and DAPI) picks up a...

06 June 2018 1,061 0 View

Alternatives to Dihydrotestosterone for cell culture?

5α-Androstan-17β-ol-3-one has a permit fee of $350 (DEA). I'm looking at effects of Estradiol on MSC and would like to investigate a male hormone aswell.

01 January 2018 4,414 1 View

Can I use multiple donors for T cell assays? Will mixing cause unwanted stimulation?

Peripheral blood mononuclear cells will be used to track proliferation via CFSE in vitro. Stimulation via PMA to investigate mechanisms of immune dampening when co-cultured with MSC's.

01 January 2016 3,496 4 View

Can I use Multiple PBMC donors for T cell proliferation?

Can I use multiple donors for T cell assays? Will mixing cause unwanted stimulation?Peripheral blood mononuclear cells will be used to track proliferation via CFSE in vitro. Stimulation via PMA to...

01 January 2016 9,789 4 View