If I have a gene with standard 18 exons and some of the exons (for example exon NO. 2, 5,7,11, and 17) were skipped in my test
how can I calculate or estimate the probable transcripts outcome or Gene variants number after exon skipping?
If there is any formula or equation to calculate the probable gene variant, it would be appreciated
To the best of my knowledge, there is no good way to predict the number of probably transcripts. You can make predictions based on information from transcriptomes, gene chips, predicted functional domains, etc.
This is even more complicated if you only have predicted intron/exon boundaries and no "wet lab" data to back it up.
Take a look in the literature to see what is already known about your gene of interest. Check the genome browsers for what is already known about possible & verified expression.
Good luck!
Hi Osama Sweef ,
I think people may be able to answer your question more easily if you specify what you mean by "some of the exons were skipped in my test". Do you mean in a wet lab experiment? Are you referring to the results of agarose gel electrophoresis? Hoping I can be more helpful with some more information.
Best,
Jessie
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