Osama Sweef

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Questions related from Osama Sweef

How can I have The promoter and Terminator sequences?

In my experimental design, I aim to track Trichosporon sp. fungus in lung tissue. To achieve this, I plan to utilize the actin gene promoter of Cryptococcus neoformans (CnPactin) and the...

22 March 2024 6,926 2 View

Alternative splicing (Exon skipped) and Gene variant estimation.......?

If I have a gene with standard 18 exons and some of the exons (for example exon NO. 2, 5,7,11, and 17) were skipped in my test how can I calculate or estimate the probable transcripts outcome or...

26 September 2022 6,335 2 View

How to estimate the intron retention (IR) level from Excel file of alternative splicing as a outcome of RNA-seq data? ?

I have received the RNAseq data outcomes as alternative splicing and differentially alternative splicing in excel files For Alternative splicing, there are data for each sample as the following ·...

24 March 2022 2,199 1 View

QPCR CT values of RN18S1 (Reference gene) show significant change between control and treated group ?

Hello, we using the same amount of RNA for cDNA synthesis, and we are sure there are no hand mistakes for doing qPCR. using both TaqMan and SYBER green master mix, the qPCR instrumentt name is...

15 November 2021 3,727 5 View

Hello my professors and my colleagues i want to ask a bout nuclear RNA quantification by qPCR ?

i isolate nuclear RNA by NORGEN protocol then i did the RT-PCR experiment 3 different time (Qiagen protocol) in qPCR results the cq valueS of nuclear RNA are low around 1-6 value i know it means...

17 March 2017 272 1 View