How to clone a promoter region of a gene within pBI101.2 vector for GUS assay?

I am cloning about 1.5kb promoter of a gene within pBI101.2 vector for GUS assay. During the primer designing, I am facing a problem. Should I include some portion(say about +20 to +30 from the...

10 June 2019 6,950 2 View

Need a protocol for SOD, Catalase and LPO enzyme assay in 96 well plate?

I need detailed protocol. Thanks in advance.

20 June 2018 6,887 3 View

What are the methods to follow if I want to study cross talk between molecular pathways?

If I want to study the cross talks between pathways involving more than 20-30 genes, what are the methods I should follow? qRT PCR and then validation by Western Blot or Immunohistochemistry is...

19 June 2018 7,134 2 View

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19 June 2018 6,121 3 View

How to plot in-plane vibration modes of square plate using MATLAB. The plot looks like a distrorted X-Y plane grid ?

I have the natural frequencies and the related displacement equations in x, y directions for in-plane vibration. Every point in the plate has a displacement in x (denoted by u(x,y) ) and y...

30 October 2017 304 11 View

How to check purity of mono-/bis-/metal complex-azo dyes using UV-Vis Spectroscopy?

How to check purity of mono-/bis-/metal complex-azo dyes using UV-Vis Spectroscopy?

22 October 2017 3,767 6 View

Given a bare-Lagrangian for a renormalizable, interacting system, is the renormaized version unique? If yes, then which ?

It is well-known that several renormalized versions of a given theory may emerge depending upon the chosen regularization method, choice of subtraction scale/scheme,...

09 March 2017 8,192 40 View

Perovskites are a class of compounda, than how can we identify that which material is suited for harvesting solar energy?

26 February 2017 4,620 3 View

Between ER and SQ grade reagents of Qualigens (Thermo fisher) which one is analytical grade [AR] reagent ?

23 November 2016 716 7 View

Why the ligand structure changes during molecular dynamics simulation?

I did MD simulation for 10 ns. the ligand was inside the simulation box upto 10ns. only in the 3rd ns the structure was ok. in all other time one of its double bond was missing. many times the OH...

23 December 2015 9,118 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View