I want to study some compounds which raises the pH to alkaline for their bactericidal action. Is there any biosensor available, or well known method to measure alkaline pH in bacteria.
Dear Sir. Concerning your issue about the method to measure alkaline pH in bacteria. The capacity of bacteria to survive and grow at alkaline pH values is of widespread importance in the epidemiology of pathogenic bacteria, in remediation and industrial settings, as well as in marine, plant-associated and extremely alkaline ecological niches. Alkali-tolerance and alkaliphily, in turn, strongly depend upon mechanisms for alkaline pH homeostasis, as shown in pH shift experiments and growth experiments in chemostats at different external pH values. Transcriptome and proteome analyses have recently complemented physiological and genetic studies, revealing numerous adaptations that contribute to alkaline pH homeostasis. These include elevated levels of transporters and enzymes that promote proton capture and retention (e.g. the ATP synthase and monovalent cation/proton antiporters), metabolic changes that lead to increased acid production, and changes in the cell surface layers that contribute to cytoplasmic proton retention. Targeted studies over the past decade have followed up the long-recognized importance of monovalent cations in active pH homeostasis. Of all the molecular determinants for growth, the hydronium and hydroxide ions are found naturally in the widest concentration range, from acid mine drainage below pH 0 to soda lakes above pH 13. Most bacteria and archaea have mechanisms that maintain their internal, cytoplasmic pH within a narrower range than the pH outside the cell, termed ‘‘pH homeostasis.’’ Some mechanisms of pH homeostasis are specific to particular species or groups of microorganisms while some common principles apply across the pH spectrum. The measurement of internal pH of microbes presents challenges, which are addressed by a range of techniques under varying growth conditions I think the following below links may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072713/pdf/nihms268778.pdf
http://biology.kenyon.edu/slonc/Micro/Cyto_pH_Ch1.pdf
Thanks
Is it possible to phrase your question in a different manner ?
Are you looking for a pH indicator ? then you can use something like a colour indicator https://www.thoughtco.com/acid-base-indicators-overview-603659. otherwise you can use pH paper , or if its liquid culture a pH electrode !
There are green fluorescent protein mutants with pH-sensitive fluorescence. Perhaps you could use that for your purpose. Here are a few references.
Article pH-Dependent Fluorescence of a Heterologously Expressed Aequ...
Article Green Fluorescent Protein as a Noninvasive Intracellular pH Indicator
https://www.caister.com/mbt/v/v2/01.pdf
You could try to mesure the activities of enzymes working in neutral cytoplasm
It may decrease with cytoplasmic alkalization induced with your compounds
Great to know about your continued dedication in your area of research...On a lighter note..You may find it near Kashmere Gate Post Office....
Here you can find good information
Article Alkaline pH Homeostasis in Bacteria: New Insights
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