Is there enough ROS formed inside cell for cidal activity ?

Increasing number of reports says that after the exposure of bacteria to bactericidal agents, there is formation of reactive oxygen species. My specific quaestion lies in the quantity of ROS, if...

01 February 2019 2,164 6 View

Can P-gp inhibitor be used to enhance the rifampicin bio-availability ?

07 August 2017 1,545 3 View

How to interpret the log cfu below detection limit ?

If one is spotting the 20 microlitre spots directly and dilutions from total 1 ml of unknown CFU sample. If one gets one or two colonies on two plate and no colonies on plate on other four plates....

31 December 2016 1,594 9 View

Why cytotoxicity for antimicrobial compounds is assessed on HepG2/J774 cell lines knowing the fact originated from cancer cells ?

Dear researchers I wish to knew the reason why researchers use cancer cell lines for assessing the cytotoxicity. Do they mimic the normal cells of human ? If yes then how ?

11 December 2016 7,569 5 View

First template cDNA buffer is missing, can make manually ?

07 August 2016 6,363 1 View

How to see cDNA first template on gel ?

Can we see whether cDNA is formed, particularly on gel. We measure the cDNA by 260/280 absorbance ratio and RNA is seen on gel. But could we see the freshly prepared cDNA first template on gel....

07 August 2016 880 6 View

Best book to study anti infective drug discovery process ?

05 June 2016 8,899 5 View

Can a biologist think of pure demarcation between vegetarian or non vegetarian ?

Is there a pure line demarcation between vegetarian and non-vegetarian ? If somebody talks of purely vegetarian, is he not eating yeast, bacteria, molds? Need only scientific input.

31 December 2015 2,127 2 View

Alkaline pH biosensor

I want to study some compounds which raises the pH to alkaline for their bactericidal action. Is there any biosensor available, or well known method to measure alkaline pH in bacteria.

01 January 1970 3,323 7 View

No title

02 March 2021 3,060 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View