My negative control cells had some damage, they are neoplastic cells and my anaplastic cell had even more damage in the negative control.

Ok, but my question is, is there much difference between the controls? For example, there was one of mine that had 6% damage in the negative control, but 17% damage in the positive control. This is weird? Wasn't the positive control supposed to have a lot of damage? Or does this not apply?

Another question, is the positive control just for us, to see if the experiment worked, or does it need to be included on the graph?

Thanks!