Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

No title

17 February 2021 4,217 3 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

Are there any papers that deal with the effects of climate change on the pollination in Tropical orchids?

There are a few papers dealing with the effects of climate change on the pollination of European orchids, but I wonder if similar studies have been carried out in the Tropics.

04 February 2021 1,818 4 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View

How do I get spectral power distribution (SPD) of my photoluminescence sample to calculate chromaticity coordinates?

Hi, I have photoluminescence samples which I already characterized and from that I already obtained the steady-state photoluminescence (SSPL) property. I want to draw CIE chromaticity diagram to...

24 January 2021 7,770 1 View

Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

20 January 2021 9,019 1 View

What is corrct way to define ports when simulating FSS and surface waves in CST?

I need to simulate a mushroom FSS and I'm struggling with the ports. What's the correct way to define them? Should I construct a proper SMA connectors, or is it enough to put a waveguide ports...

19 January 2021 6,590 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Freeze/Thaw of LS174t cells?

Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...

01 February 2021 8,723 2 View

What is the Melting point criterion in a MD simulation?

I need a quantitative criterion, something other than structural and visual qualitative features of melting in the molecular dynamics simulations.

26 January 2021 9,096 5 View

How to know homogeneity of two thermoplastic polymer composite?

I have melt mixed to thermoplastic polymers. But I want to know the homogeneity of the composite. What are the methods to determine whether the composite is homogeneous or not? Please help.

20 January 2021 9,085 5 View

How to solve melting and solidification of PCM using ANSYS Fluent?

Dear colleagues; I modeled 3D melting and solidification of phase change material filled heat exchanger and generated a (as in the picture below). First, I enabled melting and solidification...

19 January 2021 1,126 4 View

Can we re-freezing of brain tissue homogenates?

Hello! We use homogenates of mouse brains for our aims (determination of cytokines by the ELISA method). After removing the brain, we freeze the tissue in liquid nitrogen, then store it for a...

19 January 2021 5,203 1 View

How should I create (which bands) a water mask for the NDSI index based on Sentinel-2 images? and Which Modis product should I use to validate?

Hi Everyone! I do my thesis about snow melting in Austrian Alps and I have some issues. I would like to create a water mask to filter out the water bodies from the study using Sentinel 2 images....

15 January 2021 4,560 1 View