Ayman A. A.

3 Questions 7 Answers 0 Followers

Questions related from Ayman A. A.

After thawing cells, how long should I wait before freezing them again?

Is it ok to freeze cells again after less than one week of thawing them? and if not, what is the minimum time that should pass between thawing and refreezing again?

07 June 2022 5,688 1 View

How can I stain cancer spheroids using F-Actin to get a good quality images?

I tried staining 3D cancer spheroids using F-Actin stain but the F-Actin filament were not well distinguished as lines. It looked like mitochondrial or lysosomal stain. Any ideas why it turned...

06 October 2021 3,746 13 View

Why do I get high background noise and very weak signal with mitochondrial probe staining in 3D cancer spheroids?

I'm trying to stain spheroids embedded in alginate- gelatin hydrogel using mitochondria probe (not Immunostaining). However, I'm getting very high noise and very low signal with this dye...

25 August 2021 249 6 View