Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

Bacterial Contamination in Laminar Flow Hood?

Hi there! Brace yourself, this is going to be long winded and contains a year's worth of frustrations. I culture moss, and for the last year, I have been fighting a gram negative bacterial...

29 August 2020 6,492 2 View

Has anyone used Python for qualitative research analysis?

I am looking into the benefits of using programming skills for data coding and analysis. This stems from the need for highly technical skills when considering roles outside academia. If you have...

23 June 2020 9,142 1 View

Pittsburgh Sleep Quality Index Scoring Synthax on Excel?

Hi, Just checking if anyone has an Excel scoring synthax for the Pittsburgh Sleep Quality Index that they would be happy to share Thanks Kate

24 March 2020 4,047 2 View

Can I Combine Data and Tree Information into One Tree in Mesquite?

Is it possible to enter two different types of information into Mesquite? I have two sets of species data: the divergence times (in millions of years) i.e. how old each species is and the...

22 March 2020 6,304 3 View

Please how do i follow researchers on a particular field?

For instance, I'm writing on economic recession, I want to follow foreign researchers so as to be guided by their works.

27 January 2020 608 3 View

Can phenomenography be used even with limited variation in the data collected?

I am currently conducting a study and have collected interview data from students on their perspectives and experiences. I had anticipated that these experiences would be different based on their...

06 January 2020 9,667 2 View

What would be an appropriate parent-report tool for measuring circumscribed interests in young children with autism?

I'm looking to get an idea of the intensity of circumscribed interests for young children (3-6 years) with autism. Does anyone know of an appropriate parent questionnaire that can be used to...

14 January 2019 7,013 3 View

Any tips for faster EEG setup with ActiCap - particularly with kids?

I am running a study at schools and need to try to fit as many kids in a school day as possible. The EEG setup is taking a while; I already have them wash their hair with shampoo only that...

11 August 2018 1,041 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View