Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

Bacterial Contamination in Laminar Flow Hood?

Hi there! Brace yourself, this is going to be long winded and contains a year's worth of frustrations. I culture moss, and for the last year, I have been fighting a gram negative bacterial...

29 August 2020 6,492 2 View

How to estimate the bond distances in metal complexes using covalent radii?

Hello, I need to estimate the bond distances of atoms in metal complexes by using the covalent and/or van-der-Waals radii. Currently my wild guess after some thoughts is to weigh the sum of the...

13 August 2020 7,366 3 View

Has anyone used Python for qualitative research analysis?

I am looking into the benefits of using programming skills for data coding and analysis. This stems from the need for highly technical skills when considering roles outside academia. If you have...

23 June 2020 9,142 1 View

Pittsburgh Sleep Quality Index Scoring Synthax on Excel?

Hi, Just checking if anyone has an Excel scoring synthax for the Pittsburgh Sleep Quality Index that they would be happy to share Thanks Kate

24 March 2020 4,047 2 View

Can I Combine Data and Tree Information into One Tree in Mesquite?

Is it possible to enter two different types of information into Mesquite? I have two sets of species data: the divergence times (in millions of years) i.e. how old each species is and the...

22 March 2020 6,304 3 View

Please how do i follow researchers on a particular field?

For instance, I'm writing on economic recession, I want to follow foreign researchers so as to be guided by their works.

27 January 2020 608 3 View

Can phenomenography be used even with limited variation in the data collected?

I am currently conducting a study and have collected interview data from students on their perspectives and experiences. I had anticipated that these experiences would be different based on their...

06 January 2020 9,667 2 View

What would be an appropriate parent-report tool for measuring circumscribed interests in young children with autism?

I'm looking to get an idea of the intensity of circumscribed interests for young children (3-6 years) with autism. Does anyone know of an appropriate parent questionnaire that can be used to...

14 January 2019 7,013 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View