How can I know which family/species of virus is making the difference between my samples?

I have several metagenomic samples, from which I got the viromes through a bioinformatics pipeline. I made a PCoA of those samples, and some of the samples cluster together. I would like to know...

08 July 2020 8,434 2 View

Who does believe that Remdesivir has to be used early on and not (only) in advanced cases of SARS-Cov-2?

Remdesivir has been shown to speed up recovery times for patients with COVID-19 in a major US-led trial, becoming the first drug with proven benefit against the disease. Now, considering the...

01 May 2020 4,105 2 View

How can I get rid of spam on Google Scholar's "Recommended articles"?

I used to like the "Recommended articles" function in Scholar. It recommends you articles based on the citations you received (you can access them below the scholar search bar, and receive them as...

21 April 2020 6,221 7 View

Does someone know which statistical test I should use to compare these groups having a categorical dependent variable?

I have two groups (10 participants in one and 12 in the other), each one viewed 1 stimulus (web page that present 4 products to choose between). One of the web pages has an orange label under one...

31 March 2020 2,812 4 View

How can I get started with PIC code simulation?

I've already asked a question like that and followed your advice. I started studying: Plasma Physics via Computer Simulation (C K Birdsall and A B Langdon) but I really think that I need again...

21 October 2019 6,835 3 View

How to transfect murine primary bone marrow cells with retroviral vector?

Hello, I am planning to infect murine primary bone marrow cells with retroviral vectors. Does someone have experience or protocols to suggest? any kind of suggestion is really welcome! Thanks...

30 July 2019 932 3 View

How to blast a paired end fastq file?

Hello everyone, I have a paired end fastq file and I know that BLAST+ in command line, accepts fasta format. But I don't know how does it work for a paired end fastq file (I mean in two different...

22 May 2019 2,482 2 View

How do I test my (stimuli material) independent variable against the dependent variable on SPSS?

Hi all, I am currently writing my dissertation and I am struggling to analyse my survey in SPSS. I hope you can help me. I have created one survey, with 3 different stimuli material....

02 March 2019 9,851 1 View

Where can I find references about real topological vector spaces such that all vector subspaces are closed?

My interest is in characterization of spaces described in the question. Clearly the interesting case is about infinite dimensional real vector spaces.

31 January 2019 1,498 8 View

How do I remove debris from a beads suspension?

I have a suspension of 40 um beads (2 mL at 400 beads/uL) and I noticed that there are a lot of debris in it. I need to remove them as much as possible to obtain a suspension with no or few...

23 October 2016 7,569 3 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View