Adhesion problem with beas-2b?

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Vedat Şekeroğlu

Hi Tommaso,

I'm not sure that you still have the problem.

How long did you allow the cells to adhere or to proliferate following the first thawing? Did you use an attachment factor?

You must absolutely use attachment factor and should wait about 10 to 15 days after first thawing and replace the medium once in 3-4 days.

Tommaso Neri

Hi Vedat,

thaks for the informations.

Usually i don't use attachment factor, but i can try!

thanks you

Tommaso