Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

What are the important topics in the field of Artificial Intelligence in Business world?

Dear All, I am doing my MS in Electronic Businesses. And I am interested in the AI field and its applications for businesses, its benefits, impacts, etc. What kind of scientific research dominate...

26 February 2021 8,816 6 View

Why CNN algorithm performs well in Network Intrusion Detection based on some recent resources?

Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...

24 February 2021 4,578 4 View

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam?

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam? Could you provide some MATLAB code to me , a beginner for GA. Thank...

03 February 2021 3,789 5 View

How is it possible to rank variables using Likert scale?

Hello Everyone, I have a questionnaire of a 3-point Likert scale for an overall evaluation for a service, and several detailed attributes evaluation of that service. For example: Overall...

31 January 2021 9,612 8 View

Can anyone provide me with the scoring method and the questionnaire of Work life balance scale by Hayman (2005)?

Greeting! For my MA dissertation, I am thinking of taking Work life balance scale by Hayman. However, I can't find the scoring procedure; also confused about the actual rating taken. If anyone...

23 January 2021 4,074 7 View

What are the pretrained-language-model that is obviously better than BERT and RoBERTa?

19 January 2021 602 2 View

What are the easy methods or techniques to synthesize green nano particles?

because many technical steps are there to get a particular plant nano particle and its expensive too

14 January 2021 1,556 4 View

How to convert soil N% into Kg/hectare?

1. Please anyone tell me if I analyse my soil nitrogen through CHNS analyser and I get my reading in N% and according to the calculation if my reading is 0.07% then: N Kg/hectare =...

14 January 2021 7,383 11 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

How big the Balb/c mice spleen after immunization?

I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...

04 January 2021 3,591 3 View

What is the recommended/optimal time for resting mouse splenocytes after thawing?

This is my first time working with mouse cells. We isolated and froze down in 90% FCS and DMSO. I would like to retrieve them and do immunophenotyping and intracellular cytokine staining

03 January 2021 6,399 1 View

ELISPOT normalization with varying cell numbers?

Hello everyone, due to a calculation error in the spreadsheet, the splenocytes I used were wrongly diluted. As a result, the number of cells per well are identical for each mouse, but differ...

15 December 2020 9,344 1 View

How to Preserve Murine splenocytes?

Dear Researchers, I would like to know if there is a method to preserve murine splenocytes without staining for future FACS analysis for immunophenotyping? Thanks in Advance. Mohd Najib Mostafa

02 March 2020 403 2 View

Can we use trypsin EDTA on splenocytes ?

Hi, I am designing an experiment for intracellular cytokine staining. My question is, after culturing cells for 24-48 hours, can I use trypsin-EDTA on splenocytes to dettach cells? Any input will...

01 February 2020 9,471 2 View

Which medium can I used for splenocytes isolated directly from the tissue?

I want to isolate spleen cell to perform an uptake assay of nanoparticles by antigen presenting cells. The research article I refer to used phenol-free IMDM supplemented with FBS, pen-strep and...

11 December 2019 2,206 2 View

How to improve yield of MACS negative isolation (Mitenyi Biotec)?

29 October 2019 7,134 1 View

Can immortalized mast cell line (instead of splenocytes) serve as feeder cells for Tcell stimulation/expansion?

I'd like to infect an immortalized mast cell line with a virus, incubate for a day or two and then co-culture with purified CD8 T cells to induce antigen-specific activation. I see lots of...

09 October 2019 630 3 View