Acridine orange for lysosomal permeability assay in flow cytometry

02 February 2013 1 8K Report

Anyone used acridine orange for lysosomal permeability assay in cytometry? Is there a detailed protocol for me to refer to?

Robert Kiss

The attached article could maybe of help ... even if coming a little bit late ...

Best regards

Robert

Badges
Science method

More Siyuan Su's questions See All

Can GSEA (Gene Set Enrichment Analysis) be applied to a proteomics dataset?

Most of proteomic researchers abstract the biological significance of their proteomic data by examine the up/down-regulated proteins first then perform functional enrichment analysis on these...

01 February 2015 8,439 3 View

Can we export all the measured data (FCS, SSC, FITC, TRITC) for a single cell to the excel table?

I am using an Aria from BD. I want to export all the data for all the single cells to excel or txt for data analysis, not just the histogram or scatterplot. Is it possible? How to do that?

01 February 2014 9,643 2 View

Is acridine orange a proper marker for lysosomes?

My purpose was to demonstrate the lysosomal permeabilization using acridine orange. I read a few papers on the LMP, most of them suggested the red fluorescence of acridine orange is an indicator...

01 February 2014 9,109 3 View

Can anyone help with problems with statistical tests for detecting ROS using H2-DCFDA in flowcytometry?

I am trying to use flowcytometry to detect ROS using H2-DCF. Previously we have detected and compared the ROS level of treated and control cells using 96-well plate and the results are good, both...

10 November 2013 400 8 View

Can someone advise on whether to perform RNase first or RNase together with PI?

I am going to do DNA content analysis on HeLa cells and I searched for relevant protocols. Some of them suggest do PI staining and RNase A together, while others stated it was better to do RNase A...

09 October 2013 9,338 4 View

Is there any reliable software that can count cells based on image taken under a microscope?

Need to process large quantities of image. Please give me some advices. Thanks

06 July 2013 1,880 22 View

Help with 3D graphic representation of protein expression level.

I have a group of protein expression levels across three different conditions. What I want is to present the expression level of individual proteins across conditions as they are on X, Y and Z...

04 May 2013 1,805 1 View

Artificial effect of doxycycline on protein oxidation and transcription factors

In our lab, we often use doxycycline to induce gene expression or knockdown whose expression was regulated by Tet-regulated systems. This system is quite powerful and efficient until I found...

31 December 2012 8,296 6 View

Trouble with cell counting on SA beta-gal staining

I am currently doing senesecence-related beta-gal staining. The problem is I have to spend a lot of time to count the cells, which was indeed countless. Does any one have an idea about how many...

10 November 2012 3,397 3 View

Does anybody do WB to detect c-Src abundance in cells? Do you consider its solubility?

I used to use buffer with 1% NP-40, 50mM Hepes and 150mM NaCl as my lysis buffer when analyzing Src protein abundance with my samples. Since c-Src is a membrane anchored protein, I am wondering if...

05 June 2012 8,849 2 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View