Can we export all the measured data (FCS, SSC, FITC, TRITC) for a single cell to the excel table?

More Siyuan Su's questions See All

Can GSEA (Gene Set Enrichment Analysis) be applied to a proteomics dataset?

Most of proteomic researchers abstract the biological significance of their proteomic data by examine the up/down-regulated proteins first then perform functional enrichment analysis on these...

01 February 2015 8,358 3 View

Is acridine orange a proper marker for lysosomes?

My purpose was to demonstrate the lysosomal permeabilization using acridine orange. I read a few papers on the LMP, most of them suggested the red fluorescence of acridine orange is an indicator...

01 February 2014 9,033 3 View

Can anyone help with problems with statistical tests for detecting ROS using H2-DCFDA in flowcytometry?

I am trying to use flowcytometry to detect ROS using H2-DCF. Previously we have detected and compared the ROS level of treated and control cells using 96-well plate and the results are good, both...

10 November 2013 316 8 View

Can someone advise on whether to perform RNase first or RNase together with PI?

I am going to do DNA content analysis on HeLa cells and I searched for relevant protocols. Some of them suggest do PI staining and RNase A together, while others stated it was better to do RNase A...

09 October 2013 9,247 4 View

Is there any reliable software that can count cells based on image taken under a microscope?

Need to process large quantities of image. Please give me some advices. Thanks

06 July 2013 1,794 22 View

Help with 3D graphic representation of protein expression level.

I have a group of protein expression levels across three different conditions. What I want is to present the expression level of individual proteins across conditions as they are on X, Y and Z...

04 May 2013 1,711 1 View

Acridine orange for lysosomal permeability assay in flow cytometry

Anyone used acridine orange for lysosomal permeability assay in cytometry? Is there a detailed protocol for me to refer to?

01 February 2013 8,048 1 View

Artificial effect of doxycycline on protein oxidation and transcription factors

In our lab, we often use doxycycline to induce gene expression or knockdown whose expression was regulated by Tet-regulated systems. This system is quite powerful and efficient until I found...

31 December 2012 8,216 6 View

Trouble with cell counting on SA beta-gal staining

I am currently doing senesecence-related beta-gal staining. The problem is I have to spend a lot of time to count the cells, which was indeed countless. Does any one have an idea about how many...

10 November 2012 3,291 3 View

Does anybody do WB to detect c-Src abundance in cells? Do you consider its solubility?

I used to use buffer with 1% NP-40, 50mM Hepes and 150mM NaCl as my lysis buffer when analyzing Src protein abundance with my samples. Since c-Src is a membrane anchored protein, I am wondering if...

05 June 2012 8,769 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Siyuan Su

Dominic Beal, Yes, I am using a flowjo.

Thanks, I just right-clicked on the population and that's it!

Therefore I could make better images using other softwares.

Hi, John. Thanks for your tutorial. Actually we are using a BD Aria machine, which was supported by FACSDiva, but I did not pay much attention to this before.