Does anybody do WB to detect c-Src abundance in cells? Do you consider its solubility?

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Sujatha Jeyapaul

Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Siyuan Su

Thanks you all for replying. Actually I tested the solubility of Src in my own cell model. It turns out about 2/3 of total c-Src was soluble in the 1% NP-40 buffer. However, the difference in pY416Src occurs in the debris which was recovered by 2% SDS. That's amazing.