I am going to stain a cell monolayer with Methyl green pyronin. So, i need your experience regarding fixation and staining steps. Thank you
How can I add an academic journal to researchgate?
23 May 2024 8,790 1 View
Is there a difference between cognitive model of schizophrenia of beck , and rector model of functional outcome in schizophrenia,,, or the 2 model the same and can be included in one model , please
22 February 2024 1,015 1 View
I am using a new system of molecular beam epitaxy (COMPACT 21). the pressure of the growth chamber was 10^-10 torr. suddenly, we found it a round 10^-7 torr. This increase in the pressure happened...
12 February 2024 3,287 0 View
Use of auxotrophic mutants lines for transformation of fungi is a traditional practise but it does not enable future discrimination between the wild type and transgenic line. Could basta gene...
07 February 2024 8,005 2 View
How do limitations in multi-element analysis impact the comprehensive understanding of heavy metal interactions in a given environment?
18 January 2024 3,271 3 View
Is it maybe due to its hard cultivation and extraction of the toxins ? or maybe how well the fungus grow ? or even its simply because not so many fungus produce such metabolites??
13 November 2023 3,147 1 View
What is the probability of organic nitrogen transformation to ammonia in these processes partial nitrification, Anammox and aerated filter ?
20 September 2023 2,615 0 View
Comparison between Sofosbuvir free regimen and Sofosbuvir based regimens in the treatment of chronic HCV patients with chronic renal failure
03 September 2023 3,474 0 View
During the deposition copper on mild steel , the layer of coating not adhesion on the surface, I dont know which the problem
31 August 2023 6,638 2 View
Due to false positives, AI writing detectors such as GPTZero, ZeroGPT, and OpenAI's Text Classifier cannot be trusted to detect text composed by ChatGPT. What is your opinion with regard to this...
29 August 2023 3,300 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,822 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,917 0 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 588 4 View