Which is the viscosity recommended for using Methyl cellulose to perform oral gavage (mice )?

Hello, we would like to perform oral gavage in mice. The vehicle recommended for our drug consist on 0.5% methylcellulose and 5% Dimethylacetamide (0.1 ml/ 10g mouse). Could somebody help us to...

20 August 2020 8,720 2 View

Can normal colon cell lines form spheroids?

I am growing spheroids from HT29 colon cancer cells, and I need some sort of control cells to be compared with the HT29. Now, I should grow the control cell into spheroids and then use these new...

30 October 2018 3,719 12 View

Why do I have Inhibition in RNA extracted from Root bacteria ?

25 April 2017 883 6 View

New subunit E2 vaccine with celular respond after 7 days of immunization, one dose could be important?

I will appreciate very much your opinion

05 May 2016 3,366 1 View

Antibody specific for POTEF or POTEE for IHC?

I am looking for an antibody specific for POTEF or POTEE proteins (120KDa) for IHC. I have only found antibodies for FACS, WB and ELISA, and some of them are not 100% specific (bind to small...

21 April 2016 3,513 1 View

co-culturing adherent and non-adherent cells

19 April 2011 7,805 3 View

Importance and Role of Research Gate (RG) for reseachers??

RG is a medium which give chance to stay connected with experts, researchers around the world. furthermore you have easy access to read many articles for free. It is very good to become part of...

07 August 2018 4,997 9 View

Researchgate Question: How to get rid of inappropriate suggestions?

I am constantly seeing publications I am not interested in. It says "XYZ was cited in a publication". I do not even follow XYZ and I am not interested in his publications. This happens several...

07 August 2018 4,129 4 View

Is it a wrong way of thinking?

Recently someone observed that by adding a research paper his ResearchGate (RG) score increases only 0.03, while by adding a comment like "thank you", his RG increase 0.3, and he concluded based...

06 July 2018 4,556 5 View

How to add published version to the existing preprint?

Dear colleagues, If I already added a preprint (the paper was not published), and now that paper has published. So, I want to add the published version to the preprint. I don't want to add it...

06 July 2018 8,350 26 View

How to add published version to the existing preprint?

Dear colleagues, If I already added a preprint (the paper was not published), and now that paper has published. So, I want to add the published version to the preprint. I don't want to add it...

06 July 2018 2,562 19 View

How long does it take Researchgate to recognize the reference in the technical report?

I recently uploaded a technical report to Researchgate, and currently there is nothing under the 'references' tag. From my experience of uploading another technical report a few years ago, I...

05 June 2018 3,828 2 View

Why h-index and number of citations here on ResearchGate and on Google Scholar are so different?

Probably that is related to way how papers, articles and other items are entered but just wanted to make sure. Google Scholar does that automatically and also provide possibility to add articles...

05 June 2018 1,100 11 View

Do you share/talk about RG status and others to your friends on other Webs (Facebook, ...), and also your department and some places?

Do you share/talk about RG status and others to your friends on other Webs (Facebook, Twitters, ...), and also your department and some places? ...RG score....new citations...new reads...new...

03 April 2018 2,084 25 View

Why is RG score important?

The RG score or the researchgate score is calculated through some unknown algorithm. It is not proportional to the papers published and citations one earns on publications. Moreover, employers are...

03 April 2018 7,166 4 View

Did research gates web help you to achieve good collaborations?

In relation with my project : Researchers collaboration.

03 April 2018 7,025 6 View