Which is the viscosity recommended for using Methyl cellulose to perform oral gavage (mice )?

Hello, we would like to perform oral gavage in mice. The vehicle recommended for our drug consist on 0.5% methylcellulose and 5% Dimethylacetamide (0.1 ml/ 10g mouse). Could somebody help us to...

20 August 2020 8,720 2 View

Tips to quantify oxidised methionine in a full body IgG with Prot-A?

i am trying to develop a method to be able to separate oxidised and non-oxidised methionine in a full body IgG. i am using BBS ph 7 and Acetic acid with NaCl ph3. UPLC with Thermo porous...

09 December 2019 5,587 5 View

Can normal colon cell lines form spheroids?

I am growing spheroids from HT29 colon cancer cells, and I need some sort of control cells to be compared with the HT29. Now, I should grow the control cell into spheroids and then use these new...

30 October 2018 3,719 12 View

What technique to use to look for differences in two independent samples of count data?

Hi everyone, I have a sample of prices of products across different competitors. I am trying to compare the pricing across each one with the goal of looking, which company is significantly...

15 September 2018 7,980 3 View

How do I compare two companies (say two stores) using prices and two more variables in quantitative research?

07 July 2018 4,055 3 View

CFU-F assay?

I like to perform a CFU-F assay for MSC. If I understood well this takes some 2 weeks of culturing before colonies are formed. During this culture do you replace the medium or can you leave the...

04 March 2018 5,694 3 View

Why do I have Inhibition in RNA extracted from Root bacteria ?

25 April 2017 883 6 View

New subunit E2 vaccine with celular respond after 7 days of immunization, one dose could be important?

I will appreciate very much your opinion

05 May 2016 3,366 1 View

How to model a moving load in ANSYS??

Hi everyone , I need to model a moving load in ANSYS. I want to model the movement of a train on a bridge. IS there anyones who can help me with this issue??

02 May 2016 5,851 3 View

Antibody specific for POTEF or POTEE for IHC?

I am looking for an antibody specific for POTEF or POTEE proteins (120KDa) for IHC. I have only found antibodies for FACS, WB and ELISA, and some of them are not 100% specific (bind to small...

21 April 2016 3,513 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

How to correctly reference instruments in systematic review following APA?

Hello, I am running into an issue and do not know how to cite / reference correctly here. APA states that instruments and measurements need to be referenced with the author's name. At the same...

11 February 2021 9,523 3 View

Is it possible to get compass potential paramters from MATERIALS STUDIO 6.0?

Hi all, I wonder whether we can get potential parameters from material studio. I have a licensed version available. If I use polymers and cnt in materials studio to perform MD simulations,...

03 February 2021 7,673 3 View

Could you please suggest to me an interesting and good book about Nanotechnology?

I want to know about nanotechnology from beginning to professional. Thus I want some book in this area

01 February 2021 1,889 13 View

Why does the fatigue cracks initiate from surface to interior as the stress level decreases?

It is well known that low-cycle and high-cycle fatigue cracks initiate from the surface, while ultra-high cycle fatigue cracks initiate from the inside. I am very curious why this transformation...

17 January 2021 9,633 6 View

How do I write in a more professional scientific way?

I am an undergraduate in university. I am new to scientific research and would like to know how to make my paper more professional. Thanks in advance.

04 January 2021 264 12 View

What is the reason for discontinued Stage II fatigue crack growth in pearlitic steel?

Conventionally, the process of Stage II fatigue crack growth is governed by Paris law with less microstructural effect. When fatigue crack growth test was carried out for pearlitic steels ( both...

03 January 2021 7,075 3 View

What are the values of global citizenship education (GCE) from a point of view of an educator located in the Global South?

This question relates to what educators believe are today the values of global citizenship education (GCE) in the Global South. Some examples: integrity, equity, respect, fairness, empathy,...

27 December 2020 6,643 3 View