Can you recommend to me survey studies (peer-reviewed) analyzing the relationship between Blockchain and Supply Chain?

More specifically, I would like to find papers with a robust research methodology and providing the research questionnaire. Thank you.

23 November 2020 8,537 8 View

When will we have access to Brazilian agricultural censuses microdata publically available? Can Google and Microsoft help it?

Microdata are a wonderful way of analyzing specific issues in an heterogeneous environment. Brazil is wonderfully diverse country, and its countryside is also extremely heterogeneous. We have...

25 October 2020 8,900 2 View

Does anyone have a Bragg Grating simulation example in comsol?

I was searching for a bragg Grating simulation example in comsol, but I did not find any. The only one that I found was incomplete. Does anyone have a Bragg Grating simulation example in comsol?...

03 October 2020 4,337 6 View

Which is the viscosity recommended for using Methyl cellulose to perform oral gavage (mice )?

Hello, we would like to perform oral gavage in mice. The vehicle recommended for our drug consist on 0.5% methylcellulose and 5% Dimethylacetamide (0.1 ml/ 10g mouse). Could somebody help us to...

20 August 2020 8,720 2 View

Avigan ( Favipiravir), Hydroxychloroquine plus Azithromycin. A possible Cocktail against SARS-COV2 ?

All the three medicaments have promising results reported and supposed complementary mechanism of action?

10 April 2020 6,909 7 View

What might be causing the discontinuity in my UV-VIS?

The image I am posting is just two examples of gold nanoparticles, but the problem I am getting is not sample sensitive. The equipment is showing the same discontinuity no matter what we are...

23 August 2019 4,805 3 View

How to use a TEV protease from Genscript?

Hello everyone!, I would like to know if someone can help us with this issue. We are using TEV PROTEASE in my Lab following the manufacturer instructions (Genscript, USA) and we have had bad...

02 April 2019 6,248 1 View

Does anyone know any dataset of a multicriteria decision making problem?

I am looking for a dataset which contains the judgments of the decision-makers for the decision problem, as in the AHP method. The problem can be in any area. Most datasets are related with...

13 March 2019 5,469 3 View

Could we organize collective human intelligence to achieve and surpass the breakthroughs obtained or crystallized by individual human geniuses?

The biophysicist Jacques Monod hypothesized that man will never understand itself due to that a logical system could not be higher than itself. With the internet becomes common use the somewhat...

30 January 2019 8,014 10 View

Can normal colon cell lines form spheroids?

I am growing spheroids from HT29 colon cancer cells, and I need some sort of control cells to be compared with the HT29. Now, I should grow the control cell into spheroids and then use these new...

30 October 2018 3,719 12 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

No title

23 February 2021 4,014 3 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View