Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

What is the best way to differentiate L. rhamnosus and L. casei in plate co-cultivation?

I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...

13 January 2021 1,550 1 View

Have you ever included two last authors in the co-authorship of a publication?

Hello, As you must know, it is now sometimes possible to have several co-first authors in the co-authorship of a scientific publication, when two persons led the paper and had equal contribution...

21 September 2020 2,995 5 View

How does internal resistance affects the self discharge in a super capacitor?

If ESR doubles for instance, what should we expect for self discharge?

30 May 2019 3,190 0 View

Can normal colon cell lines form spheroids?

I am growing spheroids from HT29 colon cancer cells, and I need some sort of control cells to be compared with the HT29. Now, I should grow the control cell into spheroids and then use these new...

30 October 2018 3,719 12 View

Article - Research to evaluate the compaction of soils under > 2 years test ?

13 August 2018 9,973 4 View

Wich are the best combination of Restriction Enzimes for double digestion?

I want to do a RFLP assay for DNA of bacteriophages. in this case I want to performance double digestion because not all restriction enzimes cut the specific sequence of strands of DNA. thanks...

06 July 2018 4,134 1 View

Why is RFLP technology so popular for phage studies?

In many papers I see that is so popular the use of RFLP for phages studies? why not use others techniques (PCR,).

04 May 2018 8,407 0 View

Exosome Quantification

24 May 2017 3,750 3 View

Why do I have Inhibition in RNA extracted from Root bacteria ?

25 April 2017 883 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View