I am trying to make a calibration curve for Pazopanib by using HPLC. the calibration curve is coming wrong. I don't know what seems to be the problem. can anyone give me an advise?!!
You have to be precautious with the weighing of the material, standard, making of solution/stock solution and serialization. Other caution would be the reagent reactivity!
Anyway, put your queries clear enough, what was wrong with the curve?
Dear Hudasan jassim Muhammad the preparation of a calibration curve for the HPLC analysis of Pazopanib has been described in the following potentially useful research papers:
Validated RP-HPLC Method Development of Pazopanib in Bulk and its Pharmaceutical Dosage Form
https://www.phmethods.net/articles/validated-rphplc-method-development-of-pazopanib-in-bulk-and-its-pharmaceutical-dosage-form.pdf
and
HPLC–PDA ANALYSIS OF PAZOPANIB IN RABBIT PLASMA USING GEFITINIB AS INTERNAL STANDARD
https://www.ijrpc.com/files/13-10-18/06.pdf
Please find attached both articles as pdf files. I hope they are useful for you. Thank you for asking this interesting technical question! 😊
Riaz A. Khan thank you very much for your answer. The calibration curve is not linear, each time the absorbance of the IS is different and I use the same technique each time .. I honestly don't know what is the problem...
Frank T. Edelmann Thank you very much for your answer and for the links that you attached. my major problem seems to be with the IS, sometimes it has perfect absorbance and other times it is very irregular .. I will check the articles you attached
Jack Silver thank you very much for your answer. The calibration curve is not linear, each time the absorbance of the IS is different and I use the same technique each time .. I honestly don't know what is the problem
You need to check if the issue is with the instrument or the method. You use the term "IS" in your replies above - what does that mean?
- Is the retention time the same for each injection?
- Does a test compound run correctly? Are the injections repeatable?
- Are you injecting manually, or using an auto-sampler?
Jack Silver IS means internal standard, I am sure its a methodological problem
yes the retention time is the same
but the peaks of the internal standard ( gefitinib) seems to be decreasing with increasing the concentration of pazopanib, I don't know what is the explanation of that..!
You will need to explain your procedure. Assuming the HPLC procedure is stable, how are you preparing your sample?
Kindly visit..
https://www.researchgate.net/deref/https%3A%2F%2Fwww.phmethods.net%2Farticles%2Fvalidated-rphplc-method-development-of-pazopanib-in-bulk-and-its-pharmaceutical-dosage-form.pdf
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