How to increase the final DNA yield using the Qiagen PowerSoil DNA extraction kit?

Hi, I am having troubles trying to extract DNA using the Qiagen PowerSoil kit from cave sediment (mud/clay). I always get very low yield:

03 January 2021 5,049 1 View

Can all iron oxide nanoparticles be considered superparamagnetic?

Dear researchers, Can all iron oxide nanoparticles be considered superparamagnetic? Or the "superparamagnetic" definition can be assigned only to certain types of iron oxide nanoparticles? If so,...

26 October 2020 546 5 View

Do you know how long it takes for a polyprepylene surgical mask to degrade?

I'm a master student in the university of Geneva. I have to find a scientific paper of the degradation of the surgical mask to add it in my master's thesis. I only found non-scientific journals...

03 September 2020 2,940 3 View

Piecewise multiplicative scatter correction in R?

Anyone is aware of an R package available online to perform a Piecewise Multiplicative Scatter Correction (PMSC)? This technique is an advance of the Multiplicative Scatter Correction (MSC)...

23 August 2020 2,893 2 View

What is the best chromatography (type, resin..) to separate blood lipoproteins among each other?

Dear all, I am trying to understand which is the "golden standard" method to separate lipoproteins from the blood (LDL, HDL, IDL, chylomicrons...). I found some methods online, and apparently...

10 August 2020 4,738 5 View

Latent Class/Profile Analysis AND prediction of binary outcome variable?

Hey everyone, I am trying to conduct a mixture of a latent class/ profile analysis because I have categorical and continuous outcome variables. (1) how can I combine these two? I would also...

26 March 2020 354 2 View

DdRAD final library shows low concentration at size selected peak. Anyone have any idea what could have gone wrong?

I sent off my first ddRAD library for sequencing and as QC it was first run through the bioanalyzer. I size selected with a pippin prep between 375-475 bp and the results of the bionalazyer show a...

12 January 2020 9,403 1 View

Special issue on social farming. Would do you like to contribute?

Dear all, I am Valentina Cattivelli and I am a guest editor at Sustainability (IF 2.5). With this, I would like to invite you to submit a paper within a special issue "Social farming for Social...

10 November 2019 5,392 13 View

Where to find good description of a cognitive and language development test standardization?

Hello everyone, I am new in the area of Developmental Psychology and I am looking for a good description of the standardization process of a test for cognitive and/or language development. For...

28 October 2019 2,346 4 View

How to separate human from bacterial RNA?

I extracted RNA from human saliva samples but the amount of bacterial (basically not human) RNA is very high: despite the high RNA concentration (Nanodrop quantification), after real-time PCR...

22 July 2018 4,414 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View