I have been asked to develop a 3D skin cell culture model. I would like to know what are the parameters I have to consider and also I am looking for guidance, review articles and all your valuable suggestions.
Take a look at this link - you might find Alvetex 3D cell culture system a useful tool to consider. There are detailed protocols here on how to prepare a full thickness skin equivalent model using co-culture of primary human dermal fibroblasts and primary human keratinocytes. There are also some independent papers showing it's use.