Anyone please suggest a simple method to count cell suspension through UV-VIS Spectrophotometer...?
Light scattering in a UV-VIS spectrophotometer can be used to measure the "optical density" of a cell suspension, which is related to the number of cells per unit volume, but the method has to be calibrated by measuring the optical density and also counting the cells by a direct method. For bacterial cells, the measurement is often made at 600 nm. A disadvantage of this technique is that it does not distinguish between live and dead cells. If you are working with eukaryotic cells, consider using a hemocytometer and trypan blue staining instead (blue cells are dead).
https://docs.abcam.com/pdf/protocols/counting-cells-using-a-hemocytometer.pdf
UV-vis is a popular method to measure absorbance of any coloured solution. If solution is not transperent then measurement become less reliable.
Light scattering in a UV-VIS spectrophotometer can be used to measure the "optical density" of a cell suspension, which is related to the number of cells per unit volume, but the method has to be calibrated by measuring the optical density and also counting the cells by a direct method. For bacterial cells, the measurement is often made at 600 nm. A disadvantage of this technique is that it does not distinguish between live and dead cells. If you are working with eukaryotic cells, consider using a hemocytometer and trypan blue staining instead (blue cells are dead).
https://docs.abcam.com/pdf/protocols/counting-cells-using-a-hemocytometer.pdf
Hi Dr Jannathulla R.
you can be used spectrophotometer at 600 nm its very good for counting cell suspension.
regard
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