I'd like to quantify the erosion products of the polyethylene glycol (PEG) end-modified with ureidopyrimidinone (UPy) moieties.
I've read in a previous study that this can be done by measuring the UV absorbance at 265 nm. I'd like to know the reason for this specific wavelength; does it depend on the groups that compose the polymer? And how it is possible to determine it without knowing this datum in advance?
Moreover, the machine I thought to use to measure the UV absorbance is the Perkin Elmer 2030 which, however, can only measure at 260nm and 280nm. Could I use it and obtain trustable data about the erosion?
Small note: we also have a NanoDrop 1000 in our lab. Would it be worth to try with it as well?
Please look at the following below links which may help you in your analysis:
http://eprints.hud.ac.uk/id/eprint/19778/1/conwayIJP_13903.pdf https://curve.carleton.ca/system/files/etd/37e9fa73-b96f-466e-8a4d-1dbe51a2f580/etd_pdf/c73494df9c0cc903fca189ae6aed6333/aghamir-baha-measurementofpolymerconcentrationandoptimization.pdf
Thanks
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- Methodology
- Absorption Spectroscopy
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