Transgenesis

How can I achieve efficient PWPI 2nd Gen-lentivirus and primary activated T cell transduction?

Can anyone suggest methods for efficient transduction of activated primary human T cells with pWPI bicistronic vector based 2nd gen lentivirus carrying a chimeric receptor and GFP? 293T cells give...

13 August 2014 1,950 3 View

Is there a qPCR-based protocol to measure copy-number after Tol2 transgenesis in zebrafish?

There are qPCR protocols for other transposon based transgenesis vectors (e.g. SleepingBeauty: http://www.mobilednajournal.com/content/2/1/5), but I was wondering if anyone has tried it with Tol2.  

08 August 2014 9,705 2 View

Can anyone tell me where can I buy PDGFRbeta promoter?

I want PDGFRbeta promoter, I can not find it in the internet or references. Thanks.

08 August 2014 8,871 3 View

How can I achieve efficient PWPI 2nd Gen-lentivirus and primary activated T cell transduction?

Can anyone suggest methods for efficient transduction of activated primary human T cells with pWPI bicistronic vector based 2nd gen lentivirus carrying a chimeric receptor and GFP? 293T cells give...

08 August 2014 2,217 3 View

Users guide to using a Nikon/Narishige Pli 188 micro injector for injecting Drosophila embryos- can anybody help?

I have decided to do my own Drosophila injections as I can not find a company that wants to do D. virilis injections. So I got myself a Nikon Pli 188 injector on e-bay, but I don't have a clue as...

15 April 2014 7,436 1 View

Users guide to using a Nikon/Narishige Pli 188 micro injector for injecting Drosophila embryos- can anybody help?

I have decided to do my own Drosophila injections as I can not find a company that wants to do D. virilis injections. So I got myself a Nikon Pli 188 injector on e-bay, but I don't have a clue as...

04 April 2014 3,428 1 View

Do you eat transgene foods in your country?

There is a fierce controversy over food produced using gene technology (transgene food) in China currently. Some specialists insist that the transgene technology is similar to hybrid technology...

10 October 2013 3,318 0 View

Paratransgenesis for improving honeybee colony health?

Can we use microbiota for paratransgenesis in the beekeeping industry to control American foulbrood?

10 October 2013 4,232 0 View

What is the current status of attP/integrase-mediated transgenesis in Drosophila species different than melanogaster?

I am aware of such attempts ongoing at least a few years ago but haven't seen anything published yet.

08 August 2013 1,166 1 View

Protocol for Crystal Violet Gel staining, in order to isolate the DNA fragments for transgenesis?

Could anyone please let me know also the loading buffer used with Crystal Violet staining.

06 June 2013 8,650 3 View

How to achieve tol2 mediated transgenesis where only one single copy of donor DNA gets integrated into the genome?

Are there any suggestions on how I can enhance the probability to only integrate one copy of my tol2 flanked DNA fragment into the zebrafish genome? Does adding meganuclease help? I always end up...

01 January 1970 3,981 1 View

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01 January 1970 9,643 0 View

Is transposon-mediated transfection with enhanced "Sleeping Beauty" a valid method?

How do other researchers think about this method of transfection for iris and retinal pigment epithelial cells? Is it efficient, established and widely used?

01 January 1970 7,672 0 View

Contacting plant researchers based at USA…? | ResearchGate

Contacting plant researchers based at USA… Dear All: Would you please help us identify relevant research groups based in USA focused on the following areas? We are interested in research...

01 January 1970 9,799 0 View

Very recent technologies such as CRISPR / cas9: what interests and what dangers for the world and humanity? | ResearchGate

Biotech tools are evolving dramatically in the living world. Genetic transformations on the scale of the plant and animal world and the establishment of genetically modified organisms until the...

01 January 1970 2,449 0 View