Can anyone suggest the name or contact details of commercial laboratories offering chemical analysis of organic materials through LC-MS/MS?

I have plant methanol, ethyl acetate, n-hexane, and butanol extracts. I am interested in the chemical profiling of each extract through LC-MS/MS. If someone knows the details of commercial labs...

31 December 2019 1,508 4 View

Design a primer?

Hi, I am trying to design primers for attached sequence with resulting product length of 800-1000bp. But could not design any suitable primers. The tools I used are as...

10 November 2018 7,125 7 View

Are honeybees vector to different plant pathogens?

09 October 2014 9,317 9 View

How can we stain different genomes with immunofluorescence techniques?

Some insects, like rice weevil (Sitophilus oryzae), have four intracellular genomes i.e. nuclear, mitochondrial, principal endosymbiont, and Wolbachia. Can we stain these different genomes with...

10 November 2013 1,986 0 View

How can we differentiate extra cellular proteins produced by some microbes from the protein produced by an organism itself?

There are some microbes especially gurmicrobiota that perform different functions from food digestion to defence against pathogenic bacteria. They also produce some enzymes, antimicrobial peptides...

10 November 2013 7,524 4 View

Can we use immunofluorescence technique for mitochondrial DNA or RNA?

Fluorescence techniques are mostly used to identify the intracellular organs like nucleus, microtubules etc. Can we stain mitochondrial DNA or RNA from these techniques?

10 November 2013 7,814 1 View

Citation numbers differ on Research gate and Google Scholar profile

Since long I am observing that number of citations to my research publications differ on Research gate and google scholar profile. Is this happening with you also? Can anybody suggest that how can...

01 January 1970 7,279 4 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View

What is the best statistical method for analysis of pooled fecal samples?

Hello We performed a study on the intestinal microbiota of rats in six intervention groups (8 rats in each group). It is suggested that since the rats in each group were the same in terms of...

10 January 2021 2,507 4 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Fish gut microbiota extraction and identification protocol?

Hi, I need a protocol to extract and identify microbiota from fish gut. Any suggestions? Thank you. (P.s. Something low tech as I am technologically limited, guess no funds and big high tech...

01 January 2021 808 1 View

What do you suggest me as a low-cost electronic monitoring of honeybees using 3-5mg RFID tags?

We would like to control the honeybees' ID at the entrance of a flight tunnel. We would like to detect a honeybee in a cube of 10x10x10 cm3 in front of the tunnel door while flying, and thus...

17 December 2020 3,464 2 View

R script statistics - Effect of 2 variables on another?

There is data about the location and characteristics of plots of Oilseed Rape on several farms, as well as the relative abundance of different pollinator species counted at each plot. The...

25 November 2020 3,203 1 View

What is singletons in microbiota data?

I have seen in papers that many papers remove singletons from their microbiota data. I am not sure what is actually singletons and why the authors remove the singletons sequences. I would be happy...

23 November 2020 4,649 6 View

What was scientist thinking about Bees communication before Karl von Frisch discovery?

In the 1920s, Karl von Frisch pointed out that bees use special dancing patterns. What scientist or people think about bees communication before its discovery.

22 November 2020 1,426 5 View

What is the best way to prepare anaerobic medium?

Hi, I would like to prepare anaerobic medium for cultivation of gut microflora. I have an anaerobic chamber. How should I remove oxygen from the medium? Is it enough to leave it for 48 hours in...

05 October 2020 3,093 4 View

What is the best method for collecting and preparing pollen from insects stored in 70% ethanol, for visual identification?

Hello, I would like to visually identify and quantify the pollen morphotypes carried by insects which have been stored in 70% ethanol. The insects are Diptera (mainly Syrphids), and Hymenoptera...

20 August 2020 8,859 3 View