I am aware of such attempts ongoing at least a few years ago but haven't seen anything published yet.
I don't have an answer to your question, but I have been thinking about trying to make my own vectors in order to be able to do in D. virilis. So if someone else has already done so, I would be most interested.
NIG, VDRC or the TRiP (V19,V20,V22) collections is most efficient?
07 August 2013 2,696 1 View
Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...
21 April 2024 3,197 0 View
Hello team, I am using Mirdeep2 to identify miRNA in my organism (none model organism) which has no previously identified micro_RNAs. My organism is genetically close to Drosophila melanogaster....
14 April 2024 9,090 0 View
Hi all, I am using HA beads to do a Co-IP (using drosophila whole body), then do mass spec to detect the interacting partners that pulled down by HA. The one that I tried before was not good, it...
09 April 2024 2,374 0 View
Hello, Apologies if there's an obvious answer to this, I'm a first year PhD student and this task is entirely new to me. I'm including a lot of detail to convey my question clearly. In D....
04 April 2024 9,635 3 View
behavioural study of Drosophila melanogaster in DD conditions
08 February 2024 2,608 0 View
What kind of software are you using to analyze courtship behavior in Drosophila?
22 December 2023 7,302 0 View
I need a detailed protocol of SDS-PAGE only for drosophila if anyone has done before
16 November 2023 7,784 1 View
When using the UAS-GAL4 system to express the transgene in a fly (at the white prepupa stage), does altering the temperature from 25C to 18C reduce the effciency of the GAL4 driver in turn leading...
15 October 2023 5,416 4 View
The Tm of primers is 65 degrees and I am getting only non specific bands around 1kb. I amplified using Q5-HF Polymerase. How can I improve the specificity to get my desired band?
17 September 2023 4,956 5 View
I have an unusual question: I am working on a Erasmus internship project with Drosophila mutants at 2 different timepoints and with WT, KO and KI condition. A company analyzed the data using...
11 September 2023 1,743 1 View