There is a Discussion Forum created by Feng Zhang. I would advise joining that group as the technology is evolving fast (http://crispr.genome-engineering.org/).  There is two years of archived material worth purusing over.  We have been successful in making a floxed mouse using CRISPR-Cas9.  We also made the first ever regulatory edit (see ATVB paper in 2015),

There is a Discussion Forum created by Feng Zhang. I would advise joining that group as the technology is evolving fast (http://crispr.genome-engineering.org/).  There is two years of archived material worth purusing over.  We have been successful in making a floxed mouse using CRISPR-Cas9.  We also made the first ever regulatory edit (see ATVB paper in 2015),

hi Luca, for these studies I would not use an inducible CRISPR/Cas9 system as the mutation you will induce are all different in the tissue where the nuclease will be expressed. However, you can use the CRISPR/Cas9 system to create your floxed gene allele. THis line should then me crossed to an (tamoxifen) inducible Cre expresssing line (CreERT2). Alternatively this could be also done with an Tet-inducible Cre line in combination with a tissue specfic rtTA line (but somewhat more laboriours as here 4 transgenic loci are needed for a homocygous tisuue specific and inducible KO). Check first if a suitable Cre line exist. If yes, then if there are already ES cells arount (KOMP etc.) harboring a conditiona gene allele of choice. If not, go for CRISPR.

regards-

kai 

We've generated an inducible system that is compatible with existing Cas9 mice. We've not published it yet, so email me if you want to learn more. [email protected]

Front. Mol. Neurosci. | doi: 10.3389/fnmol.2016.00070

The development of a viral mediated CRISPR/Cas9 system with doxycycline dependent gRNA expression for inducible in vitro and in vivo genome editing.

Christopher A. de Solis1, Anthony Ho1, Roopashri Holehonnur1 and Jonathan E. Ploski1*

http://journal.frontiersin.org/article/10.3389/fnmol.2016.00070/abstract