I have a client that is thinking about using tagBFP2 and they are wondering how well it works in mosquitoes. Has any one used it in mosquitoes what was their experience?
Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...
10 February 2021 3,392 4 View
Hello Researchers. I am conducting a study on the determination of WNV on mosquito samples. I run nested PCR. Bands were shown to almost all samples during carrying out gel electrophoresis. After...
17 January 2021 3,263 11 View
Our transgenic mice show a really strong phenotype which differs a lot from results of another group working with the same transgene. As our mice are on a pure C57Bl6J background and theirs was on...
24 September 2020 1,792 3 View
I am interested in using a S. cerevisiae expression vector such as the pYES2-DEST52 plasmid for transgene expression in S. pombe. This plasmid has the GAL1 promotor, so I would like to make sure...
18 September 2020 7,460 2 View
During the knockout process, I found that my Salmonella was resistant to multiple antibiotics and could not screen successfully imported transgenes. Moreover, my bacteria seems to be very...
12 September 2020 6,683 1 View
Is there any study published and demonstrated which indicates the presence of the agent of rickettsioses? in other arthropods not hematophage.
02 August 2020 5,506 3 View
Is it possible that Mosquito start acting as VECTOR for all or some strains of Covid-19,if not,why? any scientific/Biological reasoning in favour or against this thought.
11 April 2020 5,260 32 View
It would be very useful to have tame strains of E. coli that are always fluorescent. So far the only ones I can find are from this work: Pinheiro, Bergquist et al. (2008), Applied Microbiology and...
09 February 2020 9,918 2 View
I would like to transfer the Dat-Cre transgene (B6 background) into a outbred strain (SW) of mice. How can this be done? Do I start with a single breeding pair (Dat-Cre B6 x SW) and then for every...
02 February 2020 4,847 4 View
01 February 2020 5,895 4 View