Has anyone used tagBFP2 in mosquitoes. We haven't screend any and a client is asking how well it works?

08 August 2017 0 7K Report

I have a client that is thinking about using tagBFP2 and they are wondering how well it works in mosquitoes. Has any one used it in mosquitoes what was their experience?

Badges
Science topic

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

What can cause that mosquitoes from Rockefeller reference strain (Aedes aegypti) spend too much time in larval stages?

We have been rearing Rockefeller mosquitoes for many years in our laboratory without any problems, but lately, in spite of good viability of eggs and unchanged environmental conditions (27°C,...

20 June 2024 3,515 2 View

Over expression in HEK293T cells?

Hello researchers, i need some help, i am packaging Lentivirus( having my transgene) in HEK293T cells, as I will collect the supernatant and concentrate my lentivirus,I also want to check...

15 June 2024 1,596 3 View

How plasmid rescue method works?

Should we use special binary vectors when using plasmid rescue method to detect transgene integration site in plant? that is, there is often no ori site between LB and RB region of simple binary...

28 May 2024 1,873 0 View

Will agrobacterium injection / infiltration techniques yield a stable transformed line?

I've recently come across the method of agroinjection and vacuum infiltration of various plant tissues (seedlings, potato eyes, tomato fruit). These methods are new to me and very fascinating but...

06 May 2024 3,036 2 View

Does the PiggyBac transposase have excision activity?

We have an immortalised human cell line that was modified using the PiggyBac transposase system to stably express luciferase. The transgene cassette contains luciferase-IRES-puro, the cells were...

27 February 2024 5,892 0 View

Can anyone provide protocol for lab culturing different species of mosquito, which is suitable for Indian Condition?

Protocol needed for culturing and lab testing

26 February 2024 8,646 1 View

Agente polinizador Aedes?

Seria interessante observar qual é o tipo de planta que o mosquito da dengue é polinizador e aplicar anti florescimento ao invés de cortar a planta ou apenas planta lá em seu centro de origem?

24 February 2024 6,543 0 View

Devise a cloning strategy to clone Chitinase gene from Spodoptera frugiperda into maize against a fungal pathogen Alternaria alternata?

If I have to make the cloning strategy, what should be my workflow to do this. I got two problems, one to select suitable expression vector from pCAMBIA1301, and pCAMBIA1302. As 1301 has GUS...

17 February 2024 8,748 2 View

Mosquito salivary glands cells number?

Hello, Does anyone have any idea of the exact (if any) number of cells we can have in mosquito salivary glands (the species doesnt matter at this stage). Thank you

05 February 2024 823 0 View