I have a construct, HyPer-mito, from the company Evrogen that I am trying to clone and use for transgenesis in zebrafish. The injected stock plasmid downstream of a cmv promoter seems to work; however, cloning HyPer-mito downstream of a tissue-specific promoter (basal skin), results in a cytosolic expression of HyPer. Multiple cloning methods have been used, and I cannot get this HyPer to localize to mitochondria. Any clues to what could be happening?

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